Abcam, ab202412, Mouse sTNF RII ELISA Kit (TNFRSF1B)

Size: 1 x 96Tests
Mouse sTNF RII ELISA Kit (TNFRSF1B) is a single-wash 90-min Simplestep used to quantify Mouse sTNF RII (TNFRSF1B) with a sensitivity of 1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum, Urine,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 1 pg/mL,
Range:6.25 - 400 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Mouse sTNF RII ELISA Kit (TNFRSF1B) (ab202412) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of sTNF RII (TNFRSF1B) protein in cit plasma, edta plasma, serum, urine, hep plasma, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse sTNF RII (TNFRSF1B) with 1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Tumor necrosis factor receptor superfamily member 1B (TNF RII) is a transmembrane protein with an extracellular domain that binds to TNF alpha. This extracellular domain can be proteolytically cleaved to make soluble TNF RII. The standard protein in this kit is soluble TNF RII and the capture and detector antibodies were raised against this region of TNF RII.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Soluble TNF Receptor II also known as TNFRSF1B or CD120b acts as a decoy receptor that binds with tumor necrosis factor-alpha (TNF-α) in the serum and extracellular space. It has a molecular mass of approximately 75 kDa. This receptor is expressed in various tissues including the immune cells and plays a role in regulating inflammatory responses. By binding TNF-α Soluble TNF Receptor II prevents TNF-α from interacting with cell surface receptors modulating its inflammatory effects.
Biological function summary
Soluble TNF Receptor II modulates inflammation and immune response. It is a part of the TNF receptor superfamily and can take part in forming a receptor-ligand complex with TNF-α impacting the downstream signaling. This receptor acts to neutralize TNF-α activity by preventing its engagement with pro-inflammatory receptors on cell surfaces. Apart from its inhibitory role it also assists in the clearance of TNF-α from the circulation managing excess TNF-α levels in the body.
Pathways
Soluble TNF Receptor II is dominant in the TNF signaling pathway and the NF-κB pathway. These pathways manage immune response cell survival and apoptosis. TNFR1 another receptor in the TNF family plays a related role in the TNF signaling pathway and often collaborates with Soluble TNF Receptor II to regulate balance between cell death and cell survival. The complex formed by Soluble TNF Receptor II and TNF-α diminishes excessive inflammation by reducing active TNF-α available for binding with TNFR1.
Soluble TNF Receptor II holds significance in rheumatoid arthritis and inflammatory bowel disease. Its presence modifies the course of these diseases by modulating the levels and effects of TNF-α. The inhibition of TNF-α-driven inflammation through interaction with Soluble TNF Receptor II can reduce tissue damage and improve symptoms. In these contexts it shows links with TNF-α which is a well-known mediator of inflammation and plays a role in the pathophysiology of these inflammatory disorders.