Abcam, ab20272, HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control

Size: 100µL
Anti-beta Actin antibody [mAbcam 8226] - Loading Control - HRP conjugated (ab20272) is a mouse monoclonal antibody detecting beta Actin in Western Blot . Suitable for Human, Mouse, Rat . - Over 290 publications - Trusted since 2005
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:mAbcam 8226,
Isotype:IgG1,
Light chain type:kappa,
Conjugation:HRP,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272) is a mouse monoclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse, Rat samples.
What is the molecular weight of beta Actin?
Anti-beta Actin [mAbcam 8226] - Loading Control HRP (ab20272) is often used as a loading control in Western blot. It specifically detects a band for beta Actin (UniProt: P60709) at a molecular weight of 42kDa.
Trusted by the scientific community
Anti-beta Actin [mAbcam 8226] - Loading Control HRP (ab20272) was first used in a scientific publication in 2005 and has been cited over 290 times in peer-reviewed journals.
Reviewed by scientists
Anti-beta Actin [mAbcam 8226] - Loading Control HRP (ab20272) has over 15 independent reviews from customers.
Related products
Antibody clone mAbcam 8226 is also available pre-conjugated to a variety of labels for your convenience – Anti-beta Actin HRP [mAbcam 8226] - Loading Control (ab20272).
Other related products
We have a range of other formats of antibody clone [mAbcam 8226] also available for your convenience:
ab8226
, HRP - ab20272, Alexa Fluor® 680 -
ab184092
, Alexa Fluor® 790 -
ab184576
, Carrier free -
ab264083

Properties and Storage Information:
Form-Liquid, Purity-IgG fraction, Storage buffer-pH: 7.4Preservative: 0.1% Proclin 300 SolutionConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Beta actin also known as beta cytoplasmic actin plays a central role in cell structure and motility. It is part of the actin protein family and is widely expressed in eukaryotic cells. The molecular weight of beta actin is approximately 42 kDa. It contributes to the formation of the cytoskeleton and participates in various cellular processes including movement and stability. Actin is abundant in all cell types providing structural integrity and flexibility.
Biological function summary
Beta actin contributes to the maintenance of cell shape and is an important player in cell division and muscle contraction. It forms part of a larger actin filaments network often associating with other proteins to form the actin cytoskeleton complex. This complex supports cellular processes such as signaling intracellular trafficking and positioning of organelles. The dynamic polymerization and depolymerization of actin filaments are critical for cellular functions.
Pathways
Beta actin functions in the regulation of important biological pathways such as the Rho/Rac/Cdc42 signaling pathway and the Wnt signaling pathway. These pathways are essential in numerous cellular activities including cell morphology and gene transcription. Beta actin closely interacts with proteins like myosin and tropomyosin which facilitate its role in muscle contraction and cell division and proteins such as Rac and Cdc42 which help govern cytoskeletal dynamics and cellular responses to extracellular stimuli.
Beta actin has links to cancers and muscular dystrophies. Aberrations in actin dynamics can result in tumor cell migration and metastasis making it a component of interest in cancer research. Additionally mutations or dysregulation in actin-associated proteins may contribute to muscular dystrophies affecting muscle function and strength. Beta actin's interactions with proteins like dystrophin involved in maintaining muscle integrity further highlight its relevance in both biological functions and disease contexts.