Abcam, ab203579, Anti-SPINK1/P12 antibody

Size: 100µL
Rabbit Polyclonal SPINK1/P12 antibody. Suitable for IHC-P and reacts with Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Rat Serine protease inhibitor Kazal-type 1 aa 50 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Rat Serine protease inhibitor Kazal-type 1 aa 50 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.P09655

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Proclin 300Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The SPINK1/P12 protein also known as serine peptidase inhibitor Kazal type 1 has a mass of approximately 11 kDa. It functions as an inhibitor of serine proteases particularly trypsin. SPINK1 is mainly expressed in the pancreas but is also present in other tissues like the gastrointestinal tract and liver. In the pancreas SPINK1 plays an important role by preventing premature activation of digestive enzymes guarding pancreatic tissue from autodigestion.
Biological function summary
SPINK1 acts as a protector in the delicate balance of enzyme regulation in tissues where it is present. It does not form part of a larger protein complex but its action is critical in reducing excessive proteolytic activity. By inhibiting trypsin SPINK1 helps avoid damage to cells that could otherwise occur if protease activity goes unchecked. This inhibition supports the stability and regulated functioning of pancreatic and gastrointestinal tissues.
Pathways
SPINK1/P12 is involved in the protease inhibition pathway and plays a role in the regulation of digestive enzyme activity specifically linked to the pancreas. SPINK1 interacts with proteins such as trypsin and other serine proteases working to suppress their activity and protect against unwanted proteolysis. This interaction helps maintain the right balance within the digestive enzyme activation pathway ensuring proper digestive processes.
SPINK1 relates closely to pancreatic disorders like hereditary pancreatitis. Mutations or deficiencies in SPINK1 can lead to an increased risk of pancreatitis due to unchecked activation of trypsin and other proteases. It is also implicated in conditions such as chronic pancreatitis. SPINK1 mutations may also affect its interaction with trypsin exacerbating the tissue damage due to enzymatic digestion.