Abcam, ab203875, Anti-PRODH antibody

Size: 100µL
Rabbit Polyclonal PRODH antibody. Suitable for IHC-P and reacts with Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human PRODH aa 150-250 conjugated to Keyhole Limpet Haemocyanin.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human PRODH aa 150-250 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.O43272

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PRODH also known as proline dehydrogenase or proline oxidase is an enzyme that plays an important mechanical role in proline metabolism. This mitochondrial protein with a mass of approximately 64 kDa catalyzes the conversion of proline to pyrroline-5-carboxylate a step important in proline degradation. PRODH is mainly expressed in several tissues including the brain heart and kidney which suggests its importance in various physiological processes.
Biological function summary
PRODH contributes to cellular energy production and redox balance by participating in proline catabolism. It operates as part of the proline utilization pathway carrying out key reactions necessary for maintaining amino acid balance and energy homeostasis. Although PRODH functions independently it collaborates with other enzymes in the mitochondrial matrix to facilitate efficient energy production and metabolic regulation.
Pathways
PRODH is involved in proline and arginine metabolism which intersects with the urea cycle and glutamate metabolism. Its activity impinges on the regulation of key pathways like the Krebs cycle through its product pyrroline-5-carboxylate which further converts to glutamate. In this context PRODH is functionally related to other enzymes such as pyrroline-5-carboxylate reductase which modulates the flow and conversion of metabolites within these pathways.
Researchers have linked PRODH to disorders like schizophrenia and cancer. Altered expression or mutations in the PRODH gene can impact metabolic flux in the brain contributing to neuropsychiatric conditions such as schizophrenia. Also the enzyme's role in proline metabolism connects it to tumorogenesis where shifts in metabolic pathways support cancer cell growth. PRODH's interactions with proteins like p53 suggest a potential mechanism for its involvement in oncogenic processes highlighting its significance in disease progression and therapeutic target potential.