Abcam, ab204001, Alexa Fluor® 488 Anti-GLUD1 + GLUD2 antibody [EPR11370]

Size: 100µL
Rabbit Recombinant Monoclonal GLUD1 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR11370,
Isotype:IgG,
Conjugation:Alexa Fluor® 488,
Excitation/Emission:Ex: 495nm, Em: 519nm,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GLUD1 and GLUD2 also known as glutamate dehydrogenases are enzymes that play roles in amino acid metabolism. GLUD1 enzyme weighs approximately 56 kDa. These enzymes convert glutamate to α-ketoglutarate and ammonia thereby regulating amino acid turnover and nitrogen metabolism. GLUD1 and GLUD2 are expressed in mitochondria across numerous tissues but are highly represented in the liver and brain tissues. Mouse GLUD1 is often studied to understand these functions through model organisms.
Biological function summary
These enzymes participate in critical processes like neurotransmitter regulation and energy production. GLUD1 and GLUD2 provide ammonia and α-ketoglutarate necessary for the Krebs cycle connecting amino acid catabolism to energy production. Although not typically part of a larger complex their activity modulates key metabolic pathways essential for cellular energy.
Pathways
GLUD1 and GLUD2 play central roles in nitrogen and tricarboxylic acid (TCA) cycles. Within these cycles they facilitate interactions with proteins like AMPK linking amino acid metabolism to cellular energy sensing. They influence energy homeostasis by modulating NADH and NADPH production further integrating with metabolic control pathways like gluconeogenesis.
GLUD1 and GLUD2 have associations with hyperinsulinism/hyperammonemia syndrome and neurodegenerative disorders such as Alzheimer’s disease. In the context of hyperinsulinism faulty regulation of GLUD1 can lead to excessive insulin production. In neurodegenerative conditions these proteins by altering neurotransmitter levels possibly interact with proteins such as amyloid-β contributing to disease pathology.