Abcam, ab204123, Anti-Adenovirus Type 5 E1A antibody

Size: 100µL
Rabbit Polyclonal Adenovirus Type 5 E1A antibody. Suitable for WB, IHC-P and reacts with Rat, Human adenovirus 5 samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human adenovirus 5 Early E1A protein aa 150-250 conjugated to Keyhole Limpet Haemocyanin.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat, Human adenovirus 5,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human adenovirus 5 Early E1A protein aa 150-250 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.P03255

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Proclin 300Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Adenovirus Type 5 E1A protein is an early viral gene product encoded by the adenovirus type 5 genome. It has a molecular mass of about 243 to 289 amino acids depending on the splice variant. This protein known for its regulatory abilities plays a central role in modulating the host cell environment to support viral replication. Expression occurs primarily in infected host cells shortly after infection. E1A proteins are necessary for the transcription of other viral genes making them pivotal in the adenovirus life cycle.
Biological function summary
The E1A protein interacts directly with cellular machinery altering the host's transcriptional and cell cycle control pathways. E1A is part of a complex that includes core cellular regulators such as p300/CBP and RB family proteins. This complex facilitates the transformation of host cells pushing them into S-phase to create an environment conducive to viral DNA replication. In addition to cell transformation E1A also modulates apoptosis enhancing virus infectivity by delaying host cell death.
Pathways
The E1A protein integrates into the broader landscape of cell cycle regulation and apoptosis pathways. It notably interacts with the retinoblastoma (RB) protein and the p53 pathway disrupting normal cell cycle checkpoints and inhibiting apoptosis. Through its interactions with RB E1A disrupts E2F transcription factor regulation leading to unchecked cellular proliferation. The p53 pathway's modulation further demonstrates how E1A contributes to creating a favorable environment for viral replication and persistence.
E1A is associated with oncogenesis due to its ability to alter cell growth and transformation processes. These interactions highlight its role in certain cancers where deregulated cell cycle control is a hallmark. E1A's interactions with the RB protein are significant in this context given RB's critical function in tumor suppression. Furthermore E1A's modulation of apoptosis relates to another disorder autoimmune conditions where disrupted cell death can lead to sustained inflammation and tissue damage.