Abcam, ab206776, Anti-beta Arrestin 1 antibody [E274] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal beta Arrestin 1 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 8 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:E274,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WB, ICC/IF, Flow Cyt (Intra), IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:The antibody immunogen shares 90% homology with ARRB2 (P32121) which has similar MW than ARRB1. Therefore, it is likely that the antbody will cross-react with ARRB2. However, we haven't performed any experiment with recombinant protein to confirm this.

Product details:
ab206776 is the carrier-free version of
ab32099
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Beta Arrestin 1 also known as β-arrestin b-arrestin or ARRB1 is a protein that plays an important role in cell signaling mechanisms. It has a molecular mass of approximately 47 kDa and is expressed in various tissues with high expression levels in the brain and testes. Beta Arrestin 1 binds to G protein-coupled receptors (GPCRs) leading to the termination of coupling between the GPCRs and G proteins. This function is essential in regulating the receptor's signal and mediating receptor desensitization.
Biological function summary
Beta Arrestin 1 functions beyond GPCR desensitization by participating in receptor internalization and the initiation of alternative signaling pathways known as arrestin-mediated signaling. It acts as part of a greater complex with clathrin and adaptin which facilitates the endocytosis of GPCRs. In doing so beta Arrestin 1 can modulate different signaling pathways independently of G proteins influencing various cellular responses.
Pathways
Beta Arrestin 1 integrates into the MAPK/ERK pathway and the PI3K/AKT pathway. These pathways are important for regulating diverse cellular processes including proliferation differentiation and survival. Through the MAPK/ERK pathway beta Arrestin 1 is connected to proteins such as extracellular signal-regulated kinases (ERK1/2). In the context of the PI3K/AKT pathway it associates with phosphoinositide 3-kinase helping to control cell growth and survival mechanisms.
Disruptions involving beta Arrestin 1 have implications in cancer and cardiovascular diseases. In cancer abnormal beta Arrestin 1 activity can influence tumor growth by altering cell proliferation and survival signaling pathways. It links to proteins like ERK1/2 and PI3K which can drive oncogenic processes. In cardiovascular disorders beta Arrestin 1 is implicated in heart failure where its altered activity affects cardiovascular signaling pathways influencing heart function and response to stress.