Abcam, ab207620, Mouse Albumin ELISA Kit

Size: 1 x 96Tests / 1 x 384Tests / 10 x 96Tests
Mouse Albumin ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Albumin with a sensitivity of 25 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Different formats for different needs: 384-well plate for higher throughput - Design your own immunoassay: we also offer the conjugation-ready antibody pair - Cited in over 15 citations
Key facts
Detection method:Colorimetric,
Sample types:Urine, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 25 pg/mL,
Range:62.5 - 4000 pg/mL,
Assay time:1h 30m,
Assay Platform:Microplate (12 x 8 well strips)

Product details:
Mouse Albumin ELISA Kit ab207620 is a rapid single-wash 90-min ELISA kit to measure Mouse Albumin in urine, serum and plasma. This SimpleStep ELISA
sensitivity is 25 pg/mL.
How the assay works
Mouse Albumin SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
This kit recognizes both native mouse Albumin protein serum, plasma and urine. Tissue and cell extract samples have not been tested with this kit.
Human serum albumin was prepared at 50 ng/mL and assayed for cross reactivity. No cross-reactivity was observed.
Mouse Albumin ELISA Kit ab207620 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Mouse Albumin ELISA kit ab207620
Mouse Albumin ELISA kit has been used in publications to study metabolic changes in cancer patients with cachexia
and effects of GLP-1 receptor agonists on kidney function
References:
Masahiro Aoki et al. 2023 PMID: 37803016,
Chi-Ming Wong et al. 2022 PMID: 35299724
Save your precious samples
Our 384-well Mouse Albumin ELISA Kit only requires a maxmum of 12.5µL
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Mouse Albumin Antibody Pair - BSA and Azide free
ab241806
- Anti-Albumin antibody [EPR18741-27] - BSA and Azide free (Capture)
ab242447
- Anti-Albumin antibody [EPR18741-67] - BSA and Azide free (Detector)
ab242698
Related and recommended products
Mouse Albumin ELISA kit ab207620 is commonly used by researchers to measure liver or kidney function.
Liver function ELISA assays include Mouse ALT ELISA kit
ab282882
and Mouse AST ELISA kit
ab263882
These ELISA kits are also available for Human and Rat:
- Human Albumin ELISA kit
ab179887
- Human ALT ELISA kit
ab234578
- Human AST ELISA Kit
ab263881
- Rat Albumin ELISA kit
ab235642
- Rat ALT ELISA kit
ab234579
- Rat AST ELISA kit
ab263883
Kidney function marker assays include:
- Creatinine Assay Kit
ab65340
- suitable for use as a serum creatinine assay and a urine creatinine assay.
- Albumin (BCG) Assay Kit (Colorimetric)
ab235628
– suitable for use with serum
- Urea Assay Kit
ab83362
– suitable for use with serum/plasma and urine, and used as a blood urea nitrogen assay.
Albumin (ALB), the main protein of plasma, has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. It also serves as a major zinc transporter in plasma, typically binds about 80% of all plasma zinc.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Albumin also known as ALB is a major plasma protein with a molecular mass of approximately 66.5 kDa. It predominantly gets synthesized in the liver and found abundantly in blood plasma. Albumin serves multiple functions including maintaining osmotic pressure and acting as a carrier protein for various endogenous substances like hormones fatty acids and bilirubin.
Biological function summary
Albumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.
Pathways
More than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.
Researchers link albumin with nephrotic syndrome and liver cirrhosis. In nephrotic syndrome low levels of albumin (albumin low) can cause edema due to imbalanced osmotic pressure. In liver cirrhosis a reduction in albumin synthesis signifies liver damage. Albumin's interaction with proteins like angiotensin II correlates with complications such as hypertension further establishing the protein's role in health and disease contexts.