Abcam, ab208195, Anti-IRS2 antibody [EPR904(2)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal IRS2 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR904(2),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, ICC/IF, Flow Cyt (Intra), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab208195 is the carrier-free version of
ab134101
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Insulin Receptor Substrate 2 (IRS2) is an important cytoplasmic adaptor protein involved in insulin signaling. Alternate names for IRS2 include IRS-2 or HEK293 products IRS2. It plays a role in transducing signals from the insulin receptor tyrosine kinase to downstream effectors. IRS2 has a molecular mass of approximately 180 kDa. Expression of IRS2 occurs in many tissues with significant presence in liver and skeletal muscle indicating its broad physiological role.
Biological function summary
IRS2 acts in signal transduction and is a part of the insulin and insulin-like growth factor signaling pathways. IRS2 does not function alone but interacts with other molecules to propagate signals that regulate cellular processes. It is essential for glucose homeostasis and lipid metabolism playing a critical role in maintaining energy balance within the organism. IRS2 also mediates other biological processes like cell growth and differentiation.
Pathways
With regards to pathways IRS2 is involved in the insulin signaling and mTOR pathways. It serves as an important junction between metabolic and growth-promoting signals. Activation of IRS2 through insulin receptors leads to downstream signaling involving PI3K and Akt essential for mediating metabolic actions of insulin. It also interacts with mTOR a central protein in cell growth regulation. These pathways illustrate the integration of IRS2 in broader cellular responses to growth signals and energy availability.
IRS2 is linked to conditions like type 2 diabetes and obesity. Dysregulation of IRS2-mediated signaling can contribute to insulin resistance a characteristic feature of type 2 diabetes. In such disorders IRS2 signaling may be impaired or altered affecting its interaction with proteins like PI3K and Akt which are also implicated in these conditions. Furthermore chronic imbalance in IRS2 activity may contribute to obesity as it affects key metabolic pathways that regulate fat storage and energy expenditure.