Abcam, ab208198, Anti-N6, N6-dimethyladenosine (m6,6A) antibody [EPR- 19831-44]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal N6, N6-dimethyladenosine (m6,6A) antibody. Suitable for IP, ELISA and reacts with Modified Nucleic Acid samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR- 19831-44,
Isotype:IgG,
Carrier free:No,
Applications:IP, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Has been developed to discriminate between the modified base N6, N6-dimethyladenosine (m6,6A) and the unmodified counterpart Adenosine (A).

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
N6 N6-dimethyladenosine (m66A) also known as MMA is a modified nucleotide found in various RNA molecules. It possesses two methyl groups at the N6 position of the adenine base contributing to the stability and processing of RNA. The molecular mass of m66A is approximately 267.27 g/mol. This modification appears in a wide range of organisms from bacteria to humans indicating its importance across species. In human cells m66A is prevalent within the cytoplasm and nucleus where it plays a role in modulating RNA behavior.
Biological function summary
M66A can affect RNA's structural configuration and its interaction with other molecules. It is a part of the broader modification network within RNAs often found with other RNA modifications such as m6A and m1A. The presence of m66A influences RNA stability splicing and export which can alter the outcome of gene expression. In the context of gene expression regulation m66A interacts with multiple proteins that recognize and modify RNA structures. Consequently it forms part of the dynamic and responsive mechanisms that control cellular activities.
Pathways
M66A participates in the RNA metabolism and gene expression pathways. It associates with critical biological functions through interactions with proteins like METTL3 and FTO which regulate RNA methylation status. These pathways are important for processes such as translation regulation and RNA decay. METTL3 a methyltransferase adds methyl groups to RNA while FTO a demethylase removes them creating a balance in the modification states essential for proper cellular function.
M66A shows connections to several conditions including cancer and metabolic disorders. Changes in m66A levels might influence cellular transformation and tumor progression linking it to oncogenic processes. Specifically altered interactions with METTL3 have been observed in certain cancers due to its role in modifying RNA and affecting gene expression programs. Additionally m66A's involvement in metabolic regulation suggests a potential link to metabolic disorders wherein disruptions in RNA modifications may lead to impaired metabolism and related diseases.