Abcam, ab2105, Anti-IL-1 beta antibody

Size: 100µg
Anti-IL-1 beta antibody (ab2105) is a rabbit polyclonal antibody detecting IL-1 beta in Western Blot, Flow Cytometry, IP, IHC-P, IHC-Fr, ICC/IF, ELISA, Neut, FuncS . Suitable for Human . - Over 190 publications - Trusted since 2000
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-FoFr, IHC-P, RIA, FuncS, Neut, IHC-Fr, Flow Cyt, WB, ICC/IF, ELISA, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human IL1B.P01584,
Specificity:In general, this antibody also detects primate IL-1 beta in the same formats using similar dilutions. The antiserum does not recognize human IL-1 alpha. In ELISA formats and other immunoreactive assays, this antibody will recognize 10% of the non-denatured (native) precursor 31,000 MW IL-1 beta containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in immunoblot analysis of natural cell products or human body fluids, the usual procedure of hearing the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1 beta precursor molecule. Denatured 31,000 precursor IL-1 beta will be recognized by this antibody but often migrates as a 35,000 MW band. This is due to the unfolding of the denatured precursor IL-1 beta exposing epitopes not exposed in the natural state. In immunoblots, depending on the number of cells, the antibody detects the 17,000 MW band in supernatants as well as a 35,000 MW band representing the 31,000 MW IL-1 beta precursor in lysates.

Product details:
What is this antibody validated in?
Anti-IL-1 beta antibody (ab2105) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA, Neutralising, Functional Studies in Human samples.
What is the molecular weight of IL-1 beta?
Anti-IL-1 beta (ab2105) specifically detects a band for IL-1 beta (UniProt: P01584) at a molecular weight of 30kDa.
Trusted by the scientific community
Anti-IL-1 beta (ab2105) was first used in a scientific publication in 2000 and has been cited over 190 times in peer-reviewed journals.
Reviewed by scientists
Anti-IL-1 beta (ab2105) has over 20 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purity-IgG fraction, Purification technique-Ion exchange chromatography DEAE-C, Purification notes-This is an IgG preparation of whole rabbit serum purified by DEAE fractionation., Storage buffer-pH: 7.2Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle