Abcam, ab211506, Anti-12 Lipoxygenase/ALOX12 antibody - C-terminal

Size: 200µL
Rabbit Polyclonal 12 Lipoxygenase/ALOX12 antibody. C-terminal. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 16 publications. Immunogen corresponding to Synthetic Peptide within Human ALOX12 aa 600-650 conjugated to Keyhole Limpet Haemocyanin.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt (Intra), WB, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human ALOX12 aa 600-650 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.P18054

Properties and Storage Information:
Form-Liquid, Purification technique-Precipitation Ammonium Sulphate, Storage buffer-pH: 7.4Preservative: 0.09% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
12-Lipoxygenase (12-LOX) also known as ALOX12 is an enzyme that catalyzes the oxygenation of arachidonic acid to produce 12-hydroxyeicosatetraenoic acid (12-HETE). It has a molecular mass of approximately 75 kDa. This enzyme is widely expressed in various tissues including platelets lungs and pancreatic tissue. By converting polyunsaturated fatty acids such as arachidonic acid into bioactive lipids 12-LOX plays an important role in regulating cellular and physiological functions.
Biological function summary
12-LOX influences many cellular processes such as inflammation and thrombosis regulation. It is part of the lipoxygenase pathway synthesizing lipid signaling molecules that modulate immune responses and cellular growth. The enzyme does not form part of a large protein complex but interacts with lipid membranes where it exerts its catalytic function. Through its activity 12-LOX contributes to the production of eicosanoids which are signaling molecules with various physiological roles.
Pathways
12-LOX is involved in the arachidonic acid metabolism pathway and the eicosanoid signaling pathway. These pathways play roles in inflammation and immune response modulation. It works alongside other related lipoxygenases such as ALOX5 which produce different but related signaling molecules. The interaction between these enzymes helps maintain a balanced production of inflammatory mediators which controls cell proliferation and responses to external stimuli.
Alterations in 12-LOX expression or function associate strongly with cardiovascular diseases and various types of cancers. In cardiovascular disease 12-LOX-derived eicosanoids contribute to the development of atherosclerosis by promoting inflammation and endothelial dysfunction. In cancer the dysregulation of 12-LOX can lead to increased tumor growth and metastasis. These conditions often exhibit altered interactions with proteins such as COX-2 another enzyme involved in eicosanoid production highlighting the role of 12-LOX in complex disease mechanisms.