Abcam, ab21286, Anti-Collagen I antibody

Size: 100µL
Anti-Collagen I antibody (ab21286) is a rabbit polyclonal antibody detecting Collagen I in Western Blot, IHC-P, IHC-Fr, ICC/IF, ELISA . Suitable for Mouse . - Over 230 publications - Trusted since 2005
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse,
Applications:ELISA, RIA, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Native Full Length Protein corresponding to Mouse Col1a1.P11087

Product details:
Anti-Collagen I antibody (ab21286) is a rabbit polyclonal antibody and is validated for use in ELISA, ICC/IF, RIA, WB in mouse samples.
Anti-Collagen I antibody (ab21286) has been cited over 230 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-Collagen I antibody (ab21286) has high sensitivity and specificity.
Anti-Collagen I (Cola1; Collagen type I) antibody (ab21286) has 16 independent reviews from customers.
Anti-Collagen I antibody (ab21286) specifically detects Collagen I (UniProt ID: P02452; Molecular weight: ~140kDa) and is sold in 100 µL selling sizes.
Collagen I, the most abundant collagen type in the human body, plays a significant role in oncology. It is a major component of the extracellular matrix (ECM) and contributes to the structural integrity of tissues. In cancer, Collagen I can influence tumor progression by promoting tumor cell proliferation, invasion, and metastasis through interactions with various cell surface receptors and signaling pathways.

Properties and Storage Information:
Purification notes-Purified by Ion exchange chromatography (DEAE-Trisacryl)., Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Collagen type I also called collagen I is a structural protein expressed mainly in connective tissues such as skin tendon bone and ligaments. It serves as an important component in providing mechanical strength and integrity to these tissues. Collagen I is a fibrillar collagen known for its triple-helix structure composed of two alpha-1 chains and one alpha-2 chain and has a molecular mass of approximately 300 kDa. Researchers often employ collagen western blot and collagen ELISA techniques for its detection. Collagen suppliers offer various collagen antibodies used in these assays to study its distribution and function.
Biological function summary
Collagen type I plays a central role in maintaining the extracellular matrix and supporting cellular environments. It interacts with other matrix proteins and cells forming complexes that help in tissue development and repair. Type I collagen is especially important in bone matrix working alongside minerals like hydroxyapatite to provide rigidity and support. Anti-collagen antibodies aid in studying its biological functions and interactions which are critical to understanding tissue dynamics.
Pathways
Collagen type I interacts with multiple signaling cascades involved in tissue remodeling and repair. It is a significant player in the TGF-β pathway which regulates fibrosis and wound healing processes. In these pathways proteins such as fibronectin and integrins work in concert with collagen type I to orchestrate cellular responses to damage. Researchers often examine its role in these pathways to uncover therapeutic possibilities for disease interventions.
Collagen type I has strong connections to conditions like osteogenesis imperfecta and fibrosis. Mutations or irregularities in collagen I production can lead to osteogenesis imperfecta a genetic disorder characterized by brittle bones. In fibrosis excessive collagen deposition disrupts normal tissue architecture contributing to organ dysfunction. In both conditions type I collagen interacts with other proteins like matrix metalloproteinases which modulate its breakdown and remodeling highlighting its importance in disease pathology.