Abcam, ab213681, Anti-MRF antibody

Size: 100µL
Rabbit Polyclonal MRF antibody. Suitable for IHC-P, WB and reacts with Rat, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human MYRF aa 450-550 conjugated to Keyhole Limpet Haemocyanin.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human MYRF aa 450-550 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.Q9Y2G1

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Proclin 300Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MRF also known as Myelin Gene Regulatory Factor is a protein that functions in regulating myelin gene expression. It is an 81 kDa protein that plays an important role in the development and maintenance of the myelin sheath in the nervous system. MRF is strongly expressed in oligodendrocytes of the central nervous system which are cells responsible for producing the myelin sheath. It acts mechanistically by activating transcription of genes associated with myelin production ensuring proper neural transmission.
Biological function summary
MRF is essential for myelination in the central nervous system. It acts as a transcription factor coordinating the expression of specific myelin-associated genes. MRF does not function alone; it operates as part of a larger transcriptional complex that involves other regulatory proteins. This complex fine-tunes the expression of myelin genes facilitating the proper assembly of the myelin sheath that insulates nerve fibers and ensures rapid signal conduction.
Pathways
MRF is important for the differentiation and maturation of oligodendrocytes. It integrates into oligodendrocyte differentiation pathways particularly involving the Wnt signaling pathway. This pathway regulates the expression of myelin-basic proteins and supports neural cell development. MRF also interacts with transcriptional proteins such as OLIG2 which are vital for the proliferation of precursor cells and subsequent myelination processes.
MRF dysfunction is linked to demyelinating diseases like multiple sclerosis and leukodystrophies. In multiple sclerosis MRF fails to activate the necessary myelin genes leading to improper myelin sheath maintenance. This disorder includes OLIG2-related disruptions affecting oligodendrocyte function and myelin production. MRF's role in leukodystrophies a group of genetic disorders affecting myelin shows how its regulatory functions are critical for proper myelin formation and neurological health.