Abcam, ab213823, Human SCGB1A1 ELISA Kit (uteroglobin)

Size: 1 x 96Tests
Human SCGB1A1 ELISA Kit (uteroglobin) is a Sandwich (quantitative) ELISA for the measurement of Human SCGB1A1 (uteroglobin) in Human in Cell Culture Media, Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant, EDTA Plasma, Heparin Plasma, Milk, Saliva, Serum, Urine,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 10 pg/mL,
Range:156 - 10000 pg/mL,
Assay time:3h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
The Human SCGB1A1 Enzyme-Linked Immunosorbent Assay (ELISA) kit (uteroglobin) (ab213823) is designed for the quantitative measurement of Human SCGB1A1 in cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk.
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for SCGB1A1/uteroglobin has been pre-coated onto 96-well plates. Standards (Expression system for standard:
E.coli
; Immunogen sequence: E22-N91) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SCGB1A1/uteroglobin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human SCGB1A1/uteroglobin amount of sample captured in plate.
SCGB1A1 (secretoglobin family 1A member 1) also known as Uteroglobin is a protein that in humans is encoded by the SCGB1A1 gene. SCGB1A1 is the founding member of the secretoglobin family of small, secreted, disulfide-bridged dimeric proteins found only in mammals. This protein is specifically expressed in Clara cells in the lungs. Numerous studies demonstrated that UG is a multifunctional protein with anti-inflammatory/ immunomodulatory properties. It inhibits soluble phospholipase A(2) activity and binds and perhaps sequesters hydrophobic ligands such as progesterone, retinols, polychlorinated biphenyls, phospholipids, and prostaglandins. In addition to its anti-inflammatory activities, UG manifests anti-chemotactic, anti-allergic, anti-tumorigenic, and embryonic growth stimulatory activities.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Uteroglobin also known as Clara cell secretory protein (CC16) is a small secreted protein with a molecular mass of approximately 16 kDa. Expressed chiefly in the respiratory epithelium of the lungs particularly by Clara cells it is also present in other tissues such as the kidney and uterus. Uteroglobin acts as an anti-inflammatory and immunomodulatory protein playing an important role in protecting epithelial cells from damage by various harmful agents.
Biological function summary
Uteroglobin serves as a multifunctional secretory agent contributing to the regulation of inflammation and tissue remodeling. It does not form part of a larger complex but acts independently. By binding and inhibiting pro-inflammatory mediators like phospholipase A2 Uteroglobin reduces the production of arachidonic acid derivatives which are involved in inflammatory processes. Additionally it acts as a chemoattractant for certain immune cells further modulating immune responses.
Pathways
Uteroglobin integrates into the immune response and inflammation pathways. It indirectly interacts with proteins such as cytokines and chemokines influencing pathways like the arachidonic acid metabolism pathway and the leukocyte extravasation signaling pathway. These interactions reduce inflammatory response and regulate immune cell movement demonstrating its anti-inflammatory function.
Low levels of Uteroglobin have been associated with respiratory conditions such as asthma and chronic obstructive pulmonary disease (COPD). By affecting inflammation pathways Uteroglobin interacts with inflammatory cytokines which exacerbate these conditions. Additionally its connection to proteins involved in these pathways makes it a potential biomarker for assessing lung injury and monitoring disease progression.