Abcam, ab214171, Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [EP197Y] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal ERK1 phospho T202 + Y204 antibody. Carrier free. Suitable for IP, Dot, ELISA, WB and reacts with Rat, Human, Mouse, Synthetic peptide samples. Cited in 7 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP197Y,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:ELISA, IP, Dot, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody detects Erk1 phosphorylated at Threonine 202 and Tyrosine 204 and Erk2 phosphorylated at Threonine 185 and Tyrosine 187.

Product details:
ab214171 is the carrier-free version of
ab76299
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Erk1 and Erk2 also known as MAPK3 and MAPK1 are part of the mitogen-activated protein kinase family. These proteins play an important role in signal transduction. Erk1 has a mass of about 44 kDa while Erk2 is slightly smaller at approximately 42 kDa. Both proteins are widely expressed in a variety of tissues contributing to the transmission of signals from the cell surface to the DNA in the cell nucleus. Specifically Erk1 and Erk2 are activated through dual phosphorylation at threonine 202 and tyrosine 204 for Erk1 and threonine 185 and tyrosine 187 for Erk2.
Biological function summary
Erk1 and Erk2 are involved in several essential cellular processes including proliferation differentiation and survival. They often operate as part of a larger complex engaging with other proteins to drive cellular responses. Upon activation they move from the cytoplasm to the nucleus where they regulate the activity of various transcription factors. Erk signaling can adjust gene expression by phosphorylating nuclear targets which influences cell cycle progression and apoptosis.
Pathways
Both Erk1 and Erk2 mainly operate within the MAPK/ERK pathway. This signaling pathway is important for transmitting signals from growth factors and other extracellular stimulants. Related proteins such as Ras and Raf are upstream activators in the pathway. Downstream Erk proteins can impact other cascades including those governing cellular growth and division. By interacting with multiple proteins within these pathways Erk1 and Erk2 ensure accurate cellular responses to environmental changes.
Alterations in the function of Erk1 and Erk2 can contribute to cancer development such as colorectal cancer and melanoma. Misregulation of Erk signaling can lead to uncontrolled cell proliferation and tumor growth. These proteins also connect to neurodegenerative disorders like Alzheimer's disease where disrupted Erk signaling may contribute to neuronal damage. Erk1 and Erk2 interact with other disease-related proteins like BRAF in cancer and APP in Alzheimer's highlighting their role in disease pathophysiology.