Abcam, ab214805, Anti-APE1 antibody [EPR4022] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal APE1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR4022,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab214805 is the carrier-free version of
ab92744
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
APE1 also known as APEX1 or apurinic/apyrimidinic endonuclease 1 functions as an important DNA repair enzyme. It plays a pivotal role in the base excision repair (BER) pathway where it recognizes and processes apurinic/apyrimidinic sites in DNA. APE1 has a molecular mass of about 37 kDa. It is expressed primarily in the nucleus with detectable levels in the cytoplasm. The expression of APE1 spans across various tissue types indicating its essential role in maintaining genomic stability.
Biological function summary
APE1 enzymatically cleaves the phosphodiester bond at abasic sites creating a nick in the DNA backbone for further repair steps. This action prevents mutations and maintains DNA integrity. APE1 also functions as a redox factor regulating the transcriptional activity of several transcription factors. It is not part of a larger complex but interacts with various BER pathway proteins such as DNA polymerase beta and XRCC1. This interaction is important for the effective repair of damaged DNA and cellular response to oxidative stress.
Pathways
APE1 is integrated within the base excision repair and redox signaling pathways. These pathways are fundamental for repairing single-strand breaks and modulating the cellular oxidative stress response. APE1 coordinates closely with other proteins like PNKP and OGG1 in the BER pathway ensuring precise and effective DNA repair. Through its redox activity APE1 influences pathways involving NF-kB and AP-1 demonstrating its multifaceted roles in cellular processes.
Alterations in APE1 function associate with cancer progression and neurodegenerative diseases. Overexpression or mutations in APE1 correlate with increased tumor resistance to chemotherapy in several cancers including lung and ovarian cancer. APE1's interaction with proteins like p53 and HMGB1 connects it to the etiology and progression of these malignancies. Furthermore impaired APE1 activity links to neurodegenerative disorders such as Alzheimer's disease where DNA repair deficiency contributes to neuronal damage and cognitive decline.