Abcam, ab215729, Anti-GSDMB antibody [EPR20841]

Size: 100µL / 1mL
Anti-GSDMB antibody [EPR20841] (ab215729) is a rabbit monoclonal antibody detecting GSDMB in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP . Suitable for Human . - Biophysical QC for unrivalled batch-batch consistency
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR20841,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:According to collaborator's data, this antibody recognizes both full-length and N-term GSDMB (PMID: 32299851).

Product details:
This antibody was developed in collaboration with Dr Feng Shao's lab, NIBS.
What is this antibody validated in?
Anti-GSDMB antibody [EPR20841] (ab215729) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP) in Human samples.
What is the molecular weight of GSDMB?
Anti-GSDMB [EPR20841] (ab215729) specifically detects a band for GSDMB (UniProt: Q8TAX9) at a molecular weight of 46kDa.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
Other related products
We have a range of other formats of antibody clone [EPR20841] also available for your convenience: ab215729, Carrier free -
ab226483
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GSDMB also known as Gasdermin-B is a protein that plays a significant role in cellular mechanisms. With a molecular weight of approximately 52 kDa GSDMB is encoded by the GSDMB gene. This protein belongs to the gasdermin family and is expressed in various tissues including the stomach esophagus and gastrointestinal tract. Its expression pattern suggests it might have specialized roles in these particular tissues.
Biological function summary
Gasdermin-B is involved in cell death processes particularly in pyroptosis which is a form of programmed cell death. GSDMB forms oligomeric complexes that create pores in the lipid bilayer of cellular membranes. These pores lead to cell swelling and lysis contributing to the inflammatory response. GSDMB also interacts with other gasdermin family proteins indicating a collaborative role in controlling cell death and inflammation.
Pathways
GSDMB is an important component of the pyroptosis pathway and the regulation of immune responses. It interacts with specific members of the caspase family especially Caspase-1 which activates Gasdermin-D in a similar pathway. GSDMB modulates inflammatory signaling by assisting in the release of pro-inflammatory cytokines through its pore-forming activity linking GSDMB to processes that maintain homeostasis and immune defense.
Gasdermin-B has been linked to various inflammatory and autoimmune conditions such as Crohn's disease and ulcerative colitis. Altered expression levels of GSDMB can influence disease severity and progression. Additionally GSDMB interacts with TNF-alpha a cytokine involved in the immune response highlighting its importance in inflammatory disease environments. Exploring GSDMB's role in these conditions can advance therapeutic strategies for treating such disorders.