Abcam, ab215987, Anti-FLI1 antibody [EPR4646] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal FLI1 antibody. Carrier free. Suitable for IHC-P, WB, ChIC/CUT&RUN-seq and reacts with Human, Mouse, Recombinant full length protein samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR4646,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Human,
Applications:IHC-P, ChIC/CUT&RUN-seq, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody does not cross-react with ERG.

Product details:
ab215987 is the carrier-free version of
ab133485
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The FLI1 protein also known as Friend leukemia virus integration 1 acts as a transcription factor. It consists of 452 amino acids resulting in a molecular mass of about 50 kDa. FLI1 is part of the ETS (E26 transformation-specific) family of transcription factors and it binds to specific DNA sequences to regulate the expression of various genes. It expresses in hematopoietic cells endothelial cells and several tissues during development. High expression levels in certain tissues suggest a significant role in diverse cellular functions.
Biological function summary
The FLI1 protein influences cell proliferation differentiation and apoptosis. It also plays a role in the regulation of genes involved in the immune response. FLI1 forms complexes with other proteins to exert its function therefore impacting gene expression in cellular contexts. Its participation in these processes indicates its regulatory impact on normal cellular development and function.
Pathways
FLI1 interacts with signaling networks that govern the hematopoietic lineage and endothelial cell function. It participates in the MAPK/ERK pathway influencing cell cycle and growth signaling. Through these pathways FLI1 has functional interactions with proteins such as GATA1 and RUNX1 establishing its importance in vascular development and hematopoiesis.
FLI1 has significant associations with Ewing sarcoma and certain leukemias. In Ewing sarcoma chromosomal translocations involve FLI1 leading to oncogenic fusion proteins that drive tumorigenesis. It also interacts with partners like EWSR1 in this context. Moreover abnormal levels of FLI1 relate to blood disorders due to its role in hematopoietic regulation. Understanding these interactions and associations aids in developing therapeutic strategies targeting these illnesses.