Abcam, ab216168, Human Mesothelin ELISA Kit

Size: 1 x 96Tests
Human Mesothelin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Mesothelin with a sensitivity of 132.5 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Saliva, Urine, Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 132.5 pg/mL,
Range:0.39 - 25 ng/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Mesothelin ELISA Kit ab216168 is a rapid single-wash 90-min Sandwich ELISA to measure Human Mesothelin in cell culture supernatant, EDTA plasma, heparin plasma, saliva, serum, urine. This SimpleStep sensitivity is 132.5 pg/mL.
How the assay works
Human Mesothelin SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human Mesothelin ELISA Kit ab216168 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-Mesothelin antibody [EPR19025-42] - BSA and Azide free (Capture)
ab242775
- Anti-Mesothelin antibody [EPR19025-45] - BSA and Azide free (Detector)
ab242775
Mesothelin is a 40 kDa GPI-anchored cell surface protein found on mesothelial cells and overexpressed in several types of cancer. The gene that encodes mesothelin is spliced into two different gene products, mesothelin and megakaryocyte potentiation factor. Mesothelin is involved in cellular adhesion, but is also secreted and normally found in several biological fluids.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C