Abcam, ab21696, Anti-DDX5 antibody

Size: 100µg
Anti-DDX5 antibody (ab21696) is a rabbit polyclonal antibody detecting DDX5 in Western Blot, ICC/IF . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Over 30 publications - Trusted since 2005
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Replenishment batches of our polyclonal antibody, ab21696 are tested in WB. Previous batches were additionally validated in ICC/IF. This application is still expected to work and is covered by our Product promise guarantee. You may also be interested in our alternative recombinant antibody, ab126730.

Product details:
What is this antibody validated in?
Anti-DDX5 antibody (ab21696) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of DDX5?
Anti-DDX5 (ab21696) specifically detects a band for DDX5 (UniProt: P17844) at a molecular weight of 69kDa.
Trusted by the scientific community
Anti-DDX5 (ab21696) was first used in a scientific publication in 2005 and has been cited over 30 times in peer-reviewed journals.
Reviewed by scientists
Anti-DDX5 (ab21696) has over 5 independent reviews from customers.
Specificity confirmed
The specificity of Anti-DDX5 antibody (ab21696) has been confirmed by Western blot testing in DDX5 Knockout HAP1 cells.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
DDX5 also known as DEAD-box helicase 5 or p68 is an RNA helicase important to various RNA processing tasks. With a molecular mass of approximately 68 kDa DDX5 unwinds RNA secondary structures using energy from ATP hydrolysis. It expresses widely in human tissues with higher levels observed in regions of active cellular proliferation. The expression pattern reflects its significant involvement in pathways that require dynamic RNA structure modifications.
Biological function summary
The function of DDX5 facilitates RNA splicing transcription and ribosome biogenesis. DDX5 is a component of various RNA processing complexes interacting with other proteins to modify pre-mRNA and other RNA molecules. It plays a role in modulating gene expression and ensuring the proper processing of precursor RNA transcripts. This makes DDX5 important in cellular differentiation and development influencing stem cell renewal and pluripotency.
Pathways
DDX5 participates in the Wnt/Β-catenin and p53 signaling pathways. In the Wnt/Β-catenin pathway DDX5 interacts with proteins such as Β-catenin to influence gene transcription involved in cell growth and differentiation. Within the p53 pathway DDX5 modulates the activity of p53 a protein known for its role in DNA damage response and apoptosis. These interactions position DDX5 at the intersection of multiple regulatory networks that govern cellular proliferation and survival.
Aberrant DDX5 expression links to cancer and neurodegenerative diseases. Overexpression or dysregulation of DDX5 has been observed in various cancers including breast cancer where it can interact with proteins such as estrogen receptor alpha promoting tumor progression. In neurodegenerative disorders particularly amyotrophic lateral sclerosis (ALS) DDX5 forms toxic aggregates with TDP-43 a protein important in RNA binding and processing. Understanding DDX5's involvement in these diseases offers insight into potential therapeutic targets and diagnostic markers.