Abcam, ab217069, Anti-UBR2 antibody

Size: 100µg
Rabbit Polyclonal UBR2 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human UBR2 aa 1700 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human UBR2 aa 1700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q8IWV8

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Purification notes-ab217069 was affinity purified using an epitope specific to UBR2 immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: 99% Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
UBR2 also known as E3 ubiquitin-protein ligase UBR2 acts as a component of the N-end rule pathway that recognizes proteins with destabilizing N-terminal residues. This recognition leads to ubiquitination and proteasomal degradation. UBR2 has a molecular mass of about 200 kDa. It is expressed in various tissues with significant expression levels found in the testes heart skeletal muscle and brain. UBR2's ability to tag proteins for degradation makes it an important player in regulating protein quality and quantity within cells.
Biological function summary
UBR2 plays an important role in the cellular processes such as meiosis and DNA damage repair. During meiosis UBR2 associates with the synaptonemal complex a critical structure for chromosome pairing and segregation. It interacts with proteins like RAD18 and influences the post-replicative DNA damage repair. In addition UBR2 participates in the turnover of proteins involved in DNA metabolic processes indicating its broad regulatory impact on genome integrity and cellular homeostasis.
Pathways
UBR2 integrates into the ubiquitin-proteasome system and the DNA repair pathways. It functions in the N-end rule pathway influencing protein degradation and consequently cell cycle and apoptosis. UBR2 interacts with other proteins such as UBR1 and ATE1 linking it to several key cellular pathways affecting growth and response to stress conditions. In the context of DNA damage response UBR2's role in promoting repair through its action on repair proteins highlights its importance in maintaining cellular stability.
UBR2 associates with infertility and cancer. Mutations or dysregulation of UBR2 can lead to reproductive issues due to its critical role in meiotic chromosome segregation aligning it with infertility concerns. Furthermore abnormal UBR2 expression or function can also contribute to tumorigenesis. It impacts the degradation of proteins like p53 a well-known tumor suppressor indicating its potential link to cancer development when pathways involving UBR2 are disrupted. Understanding UBR2's involvement in these diseases opens pathways for novel therapeutic interventions targeting its regulatory mechanisms.