Abcam, ab2179, Anti-Blooms Syndrome Protein Blm antibody

Size: 50µg
Anti-Blooms Syndrome Protein Blm antibody (ab2179) is a rabbit polyclonal antibody detecting Blooms Syndrome Protein Blm in Western Blot, IP . Suitable for Human, Mouse . - Over 40 publications - Trusted since 2002
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-Blooms Syndrome Protein Blm antibody (ab2179) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP) in Human, Mouse samples.
What is the molecular weight of Blooms Syndrome Protein Blm?
Anti-Blooms Syndrome Protein Blm (ab2179) specifically detects a band for Blooms Syndrome Protein Blm (UniProt: P54132) at a molecular weight of 159kDa.
Trusted by the scientific community
Anti-Blooms Syndrome Protein Blm (ab2179) was first used in a scientific publication in 2002 and has been cited over 40 times in peer-reviewed journals.
Reviewed by scientists
Anti-Blooms Syndrome Protein Blm (ab2179) has over 10 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7 - 8Preservative: 0.1% Sodium azide, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Blooms Syndrome Protein often abbreviated as BLM functions as a DNA helicase with a mass of approximately 141 kDa. BLM unwinds the DNA duplex important for genomic stability during replication and repair processes. This protein sometimes referred to as Bloom's Protein is expressed in various human tissues with higher levels in proliferating cells. BLM participates actively in the maintenance of genome integrity by resolving recombination intermediates and preventing crossover events.
Biological function summary
The BLM helicase acts as a critical player in the maintenance of chromosomal stability. It forms part of a complex known as the BLM complex which includes topoisomerase IIIα RMI1 and RMI2. This complex participates in homologous recombination repair ensuring proper disjunction of intertwined DNA and preventing excessive sister chromatid exchanges. This function is essential for preventing genomic instability which may lead to cell dysfunction or malignancies.
Pathways
The BLM protein associates with the DNA damage response and homologous recombination repair pathways. BLM interacts directly with components of the BRCA1-associated genome surveillance complex and indirectly with the ATM-dependent DNA damage signaling pathways to coordinate DNA repair processes. Its cooperation with proteins like RAD51 is integral for efficient DNA repair and recovery during replication stress conditions.
Bloom Syndrome directly relates to mutations or dysfunction in BLM. This rare genetic disorder leads to increased susceptibility to various cancers due to high rates of genomic instability and sister chromatid exchanges. In addition anomalies in BLM function may connect it to Werner syndrome where increased genomic instability is observed. Both conditions highlight BLM's role in safeguarding genome integrity and its critical interactions with proteins such as WRN helicase affecting cellular aging and carcinogenesis pathways.