Abcam, ab217945, Anti-c-Myb (phospho S11) antibody [EP769Y] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal c-Myb phospho S11 antibody. Carrier free. Suitable for IHC-P, IP, Dot, WB and reacts with Human samples. Cited in 13 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP769Y,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IP, WB, IHC-P, DotSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab217945 is the carrier-free version of
ab45150
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please
contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The c-Myb protein also known as Myb MYB or c-myb acts mechanically as a transcription factor that regulates the expression of target genes. It plays a role in cellular processes like proliferation differentiation and apoptosis by binding to specific DNA sequences. c-Myb has a molecular mass of roughly 75 kDa. It predominantly expresses in hematopoietic cells where it significantly influences gene transcription activity. Also it appears in some epithelial tissues indicating its versatile role in different cell types.
Biological function summary
The protein serves as a master regulator in the development of various cell types. It is not part of a traditional complex but interacts with other proteins to exert its function such as binding with co-activators or repressors to modulate gene expression. c-Myb controls the transcription of genes that are critical for cell cycle regulation and differentiation especially in the context of blood cell lineages. The protein is particularly influential in the production of red blood cells and white blood cells.
Pathways
C-Myb fits integrally into known hematopoietic signaling cascades and the Wnt signaling pathway. It works alongside other proteins like cyclin-dependent kinase (CDK) family members and transcription factor GATA-1 to modulate lineage-specific gene expression. Through its regulatory actions c-Myb influences the activation of downstream effectors that propagate signals essential for maintaining cellular functions within these pathways. The modulation of these pathways underlines its role in cell fate decisions.
Studies link c-Myb to several forms of leukemia and colon cancer. In leukemia aberrant expression of c-Myb disrupts normal hematopoiesis contributing to the uncontrolled proliferation of immature blood cells. The abnormal interactions between c-Myb and proteins like Bcl-2 can lead to enhanced cellular survival further promoting malignant conditions. In colon cancer c-Myb overexpression influences tumor growth and progression often acting alongside other oncogenic factors to enhance disease severity.