Abcam, ab218546, Anti-Zika Virus NS1 antibody [B4]

Size: 100µg
Mouse Monoclonal POLG antibody. Carrier free. Suitable for WB, sELISA and reacts with Zika virus samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Zika virus Genome polyprotein.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:B4,
Isotype:IgG1,
Carrier free:Yes,
Reacts with:Zika virus,
Applications:WB, sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Zika virus Genome polyprotein.Q32ZE1,
Specificity:ab218546 is specific for the NS1 protein of Zika virus, detecting NS1 from both the Uganda and Suriname strains. It demonstrates negligible cross-reactivity with NS1 proteins from Dengue virus (all serotypes), Japanese Encephalitis Virus and Yellow Fever Virus. A small amount of cross-reactivity has been observed with the NS1 protein from West Nile Virus in a direct ELISA.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-ab218546 was purified from hybridoma cell culture supernatant by affinity chromatography on Protein G. >95% pure by SDS-PAGE., Storage buffer-pH: 7 - 8Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Zika Virus NS1 also called non-structural protein 1 plays an important role in the viral life cycle. This glycoprotein has an approximate mass of 46-55 kDa and is highly expressed in cells infected by the Zika virus. NS1 is secreted into the extracellular space and found in sera of infected individuals. It exists in different forms with distinct intracellular and extracellular implications displaying versatility in its function and critical role during infection.
Biological function summary
Zika Virus NS1 plays essential roles in immune system evasion and viral replication. NS1 influences the viral pathogenesis through interactions with host immune responses helping the virus avoid recognition. This protein is part of the viral replication complex and contributes to the integrity of viral RNA. The ability of NS1 to modulate host cellular processes enhances the Zika virus's capacity to proliferate and survive within the host.
Pathways
Zika Virus NS1 is central in both evading immune defenses and assisting viral replication. It modulates innate immune pathways including interferon responses limiting host defenses. Through these pathways NS1 interacts with several proteins such as the Toll-like receptors which are critical for detecting viral infections. Additionally NS1 cooperates with other viral non-structural proteins to sustain efficient replication processes.
Zika Virus NS1 is linked to congenital Zika syndrome and Guillain-Barré syndrome. NS1 presence in circulation associates with the virus's ability to traverse the placental barrier leading to severe outcomes like microcephaly in newborns. The interactions between NS1 and host proteins such as complement factors further impact the disease progression and severity complicating immune system responses during infection. Understanding NS1's role enhances our ability to diagnose prevent and treat Zika virus-related conditions.