Abcam, ab219741, HRP Anti-CREB (phospho S133) antibody [E113]

Size: 100µL
Rabbit Recombinant Monoclonal CREB phospho S133 antibody - conjugated to HRP. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:E113,
Isotype:IgG,
Conjugation:HRP,
Carrier free:No,
Reacts with:Human,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody is specific for CREB phosphorylated on Serine 133. The immunogen of the antibody shares 94% homology with CREM (S136) and 86% homology with ATF1 (pS63). No experiment has been performed to verify the possible cross-reactivity.This antibody can't detect signal in mouse and rat brain related tissues.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.1% Proclin 300 SolutionConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CAMP response element-binding protein better known as CREB functions as a transcription factor that regulates gene expression. CREB protein operates by binding to specific DNA sequences called cAMP response elements in the promoters of target genes leading to increased or altered transcription. The protein has a molecular weight around 43 kDa. Expressed broadly in many tissues CREB's activity is particularly high in the brain where it plays key roles in neuronal functions. Researchers also refer to phospho-CREB when discussing the activated form of this protein often analyzed through methods like CREB Western blot.
Biological function summary
CREB contributes significantly to cellular processes such as proliferation differentiation and survival through transcriptional regulation. CREB does not function alone; it frequently associates with other proteins forming complexes to exert its regulatory roles. Additionally CREB influences the development of memory and learning in neurons highlighting its extensive involvement in neurophysiological processes.
Pathways
CREB plays a substantial role in the cAMP signaling pathway as well as the mitogen-activated protein kinase (MAPK) pathway. Through its interaction with kinases and other signaling proteins CREB influences numerous downstream targets affecting cellular responses to external stimuli. Notably protein kinase A (PKA) activates CREB by phosphorylation facilitating its role in various signal transduction pathways and linking it to downstream transcriptional events.
Research links CREB to neurological disorders and some forms of cancer. Aberrant CREB activity associates with neurodegenerative diseases like Alzheimer’s where dysregulated gene expression impacts neuronal viability and function. Furthermore in cancer CREB's role in cell proliferation and survival ties it to oncogenes such as BCL2 where abnormal CREB function can promote tumorigenesis. Understanding these connections provides valuable insights for therapeutic strategies against such diseases.