Abcam, ab219827, Anti-MCU antibody [CL3576]

Size: 100µL
Mouse Monoclonal MCU antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MCU aa 250-350.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:CL3576,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human,
Applications:ICC, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human MCU aa 250-350. The exact immunogen used to generate this antibody is proprietary information.Q8NE86,
Epitope:Binds to an epitope located within the peptide sequence RDRQYLLFFH as determined by overlapping synthetic peptides.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The mitochondrial calcium uniporter (MCU) is an ion channel located in the inner mitochondrial membrane. It functions to facilitate the uptake of calcium ions (Ca2+) into the mitochondria. MCU is sometimes referred to by its alternate names such as mitochondrial calcium uptake1 (MICU1). The molecular weight of MCU is approximately 40 kDa. This protein is expressed in various human tissues with higher levels observed in the heart brain and skeletal muscles.
Biological function summary
The MCU plays a critical role in regulating mitochondrial calcium levels which affect cellular energy production metabolism and apoptosis. MCU is part of a larger protein complex that includes essential regulatory subunits like MICU1 and MICU2. These subunits help maintain precise calcium ion transport into the mitochondria ensuring cellular homeostasis. Under normal conditions MCU controls energy metabolism by regulating ATP production through the mitochondrial electron transport chain.
Pathways
MCU participates in the calcium signaling pathway which links intracellular calcium levels to various metabolic responses. This protein influences the electron transport chain by affecting the activity of enzymes such as FADH2 and NADH which are important in oxidative phosphorylation. Additionally MCU interacts with key regulatory proteins like MICU1 playing a role in adjusting calcium uptake based on the cell’s metabolic needs. These interactions demonstrate MCU's integration with cellular energy pathways and its effect on maintaining metabolic balance.
Researchers have linked dysfunction of MCU to certain neurological disorders such as Parkinson's disease where impaired calcium handling leads to neuronal cell death. Additionally abnormal MCU activity may contribute to the development of cardiac diseases as calcium misregulation affects heart muscle contraction and energy demand. MCU's relationship with these diseases often involves other mitochondrial proteins influencing pathways central to the disease mechanisms. For instance its interaction with MIUC1 and ATP synthase in the mitochondrial pathways has been seen to significantly impact cardiac cellular health.