Abcam, ab219953, Anti-Cleaved PARP1 antibody [Y34] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal PARP1 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 15 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:Y34,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:Flow Cyt (Intra), IP, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody is specific for p85 cleaved form of PARP1.

Product details:
ab219953 is the carrier-free version of
ab32561
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please
contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purity-IgG fraction, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cleaved PARP1 also known as cPARP is a fragment of the PARP1 protein an important DNA repair enzyme. The full PARP1 protein has a molecular weight of approximately 116 kDa but after cleavage during apoptosis the cleaved PARP1 fragments typically have a molecular weight of around 89 kDa and 24 kDa. PARP1 is expressed abundantly in the cell nucleus where it plays important roles in maintaining genomic integrity. The cleavage of PARP1 is a common marker for cell apoptosis pointing towards its breakdown in response to cellular stress.
Biological function summary
The enzymatic function of PARP1 involves the transfer of ADP-ribose units from NAD+ to target proteins a process known as ADP-ribosylation. PARP1 operates as a part of the base excision repair complex essential in DNA repair processes. The cleaved form of PARP1 no longer facilitates DNA repair marking a shift towards apoptosis. When PARP1 is cleaved it indicates caspase activity implying cells are undergoing programmed cell death.
Pathways
Cleaved PARP1 is deeply involved in the apoptosis and DNA damage response pathways. In the apoptosis pathway PARP1 interacts with key proteins like caspase-3 which cleaves PARP during apoptosis. In the DNA damage response PARP1 collaborates with proteins such as XRCC1 facilitating the base excision repair pathway important for fixing single-strand DNA breaks. These pathways highlight the dual role of PARP1 in promoting cell survival through repair and cell death via apoptosis.
Cleaved PARP1 serves as an important marker in cancer and neurodegenerative diseases. In cancer research elevated levels of cleaved PARP1 suggest increased rates of apoptosis in response to anti-cancer therapies linking it to tumor suppression efforts. In neurodegenerative diseases excessive activation and cleavage of PARP1 can result in cell death exacerbating conditions like Alzheimer's disease. Through these contexts cleaved PARP1 connects to other therapeutic targets such as caspase proteins in cancer and to potential PARP inhibitors in neurodegenerative disorders.