Abcam, ab220669, Anti-CAPZB antibody

Size: 100µL
Rabbit Polyclonal CAPZB antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human F-actin-capping protein subunit beta aa 100-250.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human F-actin-capping protein subunit beta aa 100-250. The exact immunogen used to generate this antibody is proprietary information.P47756

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CAPZB also known as the capping protein beta subunit is an essential component of the actin cytoskeleton. It has a molecular mass of about 31 kDa and is expressed ubiquitously across various human tissues. CAPZB partners with CAPZA to form a heterodimeric complex called the capping protein which binds to the barbed ends of actin filaments. This binding is essential for regulating actin filament growth and stability by preventing the addition and loss of actin monomers at these ends.
Biological function summary
The capping of actin filaments by CAPZB helps maintain cellular structures and enables extensive processes like cell motility. CAPZB is an important part of the actin-capping activity within the capping protein complex. It directly affects processes such as muscle contraction cell migration and cell adhesion by modulating actin dynamics. Through these functions CAPZB impacts various cell types including muscle cells and neurons by ensuring proper assembly and function of actin networks.
Pathways
CAPZB plays a prominent role in the regulation of actin-associated pathways including the Arp2/3 complex pathway and the WAVE regulatory complex pathway. These pathways are involved in the control of actin nucleation and branching which are vital for reshaping the actin cytoskeleton in response to cellular signals. CAPZB interacts with proteins such as profilin and cofilin which are important for actin polymerization and depolymerization dynamics in these pathways.
CAPZB has shown links to cardiac hypertrophy and neurological disorders like Alzheimer's disease. Aberrations in CAPZB function can lead to disrupted actin dynamics affecting cardiac tissue structure and neuron morphology. In cardiac hypertrophy CAPZB's interactions with sarcomeric proteins like troponin are important while in Alzheimer's disease altered CAPZB function may connect with tau protein modifications impacting the cytoskeletal network within neurons.