Abcam, ab221161, Anti-Rho A + B + C antibody [EPR18299] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal RHOA antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR18299,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, WB, Flow Cyt (Intra), IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab221161 is the carrier-free version of
ab188103
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Rho proteins including Rho A B and C are small GTPases and part of the larger Rho family sometimes referred as Ras homologous proteins. They have a mass of approximately 21 kDa and play an important role in actin cytoskeleton dynamics. These proteins are primarily expressed in various tissues but are especially prevalent in the brain heart and lungs. When activated Rho proteins bind to GTP facilitating the reorganization of actin fibers critical for processes like cell movement and division.
Biological function summary
Rho proteins influence cell shape migration and adhesion. They are part of the Rho GTPase family which interacts with other molecules to form a complex that manages cellular structure. Rho proteins impact the formation of stress fibers and focal adhesions which are essential for maintaining tissue integrity and facilitating cellular responses. This activity makes Rho proteins vital players in wound healing and embryonic development.
Pathways
Rho proteins are critical parts of the Rho-ROCK (Rho-associated protein kinase) pathway and the Wnt signaling pathway. Both pathways are important for cell signaling that orchestrates changes in the cytoskeleton and cell behavior. In these pathways Rho proteins interact with kinases like ROCK1 and ROCK2 connecting to a larger network of signaling proteins that guide cellular outcomes involved in proliferation and structural maintenance.
Rho proteins have connections to cancer progression and cardiovascular diseases. Their role in cell adhesion and migration links them to metastasis where their dysregulation can promote tumor invasion. Also in cardiovascular conditions altered Rho activity impacts vascular smooth muscle contraction and endothelial function. Research associations identify proteins like E-cadherin in cancer and vascular endothelial cadherin in heart diseases as being influenced by Rho protein signaling.