Abcam, ab221547, Anti-ZO1 tight junction protein antibody [EPR19945-296]

Size: 20µL / 100µL / 1mL
Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547) is a rabbit monoclonal antibody detecting ZO1 tight junction protein in Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF . Suitable for Human, Mouse, Rat . - Biophysical QC for unrivalled batch-batch consistency - Over 70 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR19945-296,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat, Human, Mouse,
Applications:ICC/IF, IHC-P, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Product Specifications
Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P in human, mouse, rat samples.
Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547) specifically detects ZO1 tight junction protein (UniProt ID: Q07157; Molecular weight: 195kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).
Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547) has been cited over 75 times in peer reviewed journals and is trusted by the scientific community.
Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547) has 6 independent reviews from customers.
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Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle