Abcam, ab222065, Anti-POLR1A antibody

Size: 100µL
Rabbit Polyclonal POLR1A antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human POLR1A aa 800-950.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human POLR1A aa 800-950. The exact immunogen used to generate this antibody is proprietary information.O95602

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The POLR1A protein also known as RNA polymerase I subunit RPA1 plays an important mechanical role in the synthesis of ribosomal RNA (rRNA). POLR1A has a molecular mass of approximately 194 kDa. This protein is ubiquitously expressed across various tissues contributing extensively to the cellular machinery for protein synthesis. As an essential component of RNA polymerase I POLR1A shares structural features typical of RNA polymerase enzymes facilitating the transcription of rRNA genes.
Biological function summary
POLR1A functions as part of the RNA polymerase I complex which specializes in transcribing rRNA a critical component of ribosome biogenesis. This process is central to the synthesis of rRNA precursors which later modify to form mature rRNA. POLR1A works in conjunction with other subunits to ensure efficient and accurate transcription within the nucleolus demonstrating its significance in maintaining cellular protein synthesis capacity. The protein's activity supports the assembly of ribosomes influencing cell growth and division.
Pathways
POLR1A is integrally involved in the ribosome biogenesis and rRNA transcription pathways. These pathways are essential for producing ribosomes the molecular machines responsible for protein translation in cells. POLR1A has interactions with proteins like TIF-IA and UBF both of which regulate its transcriptional activity at specific rRNA gene promoters. This interaction regulates POLR1A's function in response to cellular conditions ensuring that protein synthesis matches cellular demands.
POLR1A's function has links to conditions like Treacher Collins syndrome and Acrofacial Dysostosis. Mutations in POLR1A can lead to defects in ribosome biogenesis disrupting the growth and development of craniofacial structures observed in these syndromes. Additionally POLR1A interacts with proteins such as NOP56 and fibrillarin which are involved in rRNA processing and assembly suggesting that any disruptions in these interactions could contribute to ribosomopathies. POLR1A's role in maintaining rRNA transcription highlights its significance in preventing defects that translate to broader systemic issues.