Abcam, ab223066, Anti-ML-1 antibody

Size: 50µL
Rabbit Polyclonal ML-1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ATP8A2 aa 350-600.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human ATP8A2 aa 350-600. The exact immunogen used to generate this antibody is proprietary information.Q9NTI2

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes->95% pure, Storage buffer-pH: 7.4Preservative: 0.03% Proclin 300Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ML-1 also known as Mucolipin-1 is a protein encoded by the MCOLN1 gene. It functions as a non-selective cation channel involved in transporting ions across the lysosomal membrane. With a molecular mass of approximately 65 kDa ML-1 mostly expresses in tissues like heart kidney and brain. This protein facilitates the release of calcium ions from the lysosome into the cytoplasm playing an important role in lysosomal homeostasis and cellular processes.
Biological function summary
The function of ML-1 influences endolysosomal trafficking and autophagy. ML-1 is a part of a larger TRPML channel family working collaboratively to ensure proper lysosomal storage and degradation activities. It modulates interactions between lysosomes and other cellular organelles impacting membrane repair and cellular metabolism. Dysfunctional ML-1 can disrupt these processes causing significant cellular malfunctions.
Pathways
ML-1 takes part in the lysosomal exocytosis pathway and calcium signaling pathways. These pathways play key roles in cellular nutrient sensing and membrane fusion events. ML-1 collaborates with proteins such as TRPML2 and TRPML3 which are also members of the TRPML channel family. Together they contribute to fine-tuning calcium signals inside cells supporting diverse biological functions tied to cellular maintenance and repair.
ML-1 mutations associate closely with mucolipidosis type IV a condition affecting the nervous system and eyes. This disorder arises from impaired lysosomal function due to faulty ML-1 activity. Additionally research connects ML-1 malfunction with neurodegenerative diseases linking it to abnormal accumulation of proteins like APP seen in Alzheimer's disease. Dysfunctional ML-1 and its interaction with such proteins illustrate its significance in maintaining cellular health and highlights potential therapeutic targets for related disorders.