Abcam, ab223138, Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Glucocorticoid Receptor antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Transfected cell lysate - Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR19621,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-P, ICC/IF, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab223138 is the carrier-free version of
ab183127
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The glucocorticoid receptor (GR) also known as the cortisol receptor is a type of nuclear receptor that functions as a transcription factor. This receptor has a molecular mass of approximately 97 kDa. GR is expressed in various tissues including the liver lung and immune cells. Its mechanical action involves binding to glucocorticoids—hormones like cortisol—which can regulate DNA transcription. When activated the receptor translocates into the cell nucleus where it can directly interact with DNA to regulate gene expression.
Biological function summary
The glucocorticoid receptor plays a significant role in mediating the physiological effects of glucocorticoids. It controls processes like inflammation and immune response. The receptor is part of a larger receptor complex enhancing its effectiveness in gene regulation. It modulates expression levels of diverse genes involved in metabolism immune functionality and cellular growth. GR's regulatory actions make it contextual within various biological processes impacting cellular behavior extensively.
Pathways
The glucocorticoid receptor integrates into significant signaling networks like the hypothalamic-pituitary-adrenal (HPA) axis and the inflammatory response pathway. This receptor coordinates with other proteins to control stress responses and inflammatory signals. In the HPA axis GR helps regulate cortisol levels and counteracts inflammatory cytokines. Its interaction with other receptors and transcription factors exemplifies its role in essential pathways that maintain homeostasis and stress adaptation within the organism.
Abnormal glucocorticoid receptor function links to conditions like Cushing's syndrome and glucocorticoid resistance. Cushing's syndrome characterized by excessive cortisol levels shows altered GR signaling. Similarly glucocorticoid resistance involves mutations or dysfunctions in GR that lead to improper hormone action affecting inflammation and immune responses. These diseases often involve other proteins such as various cytokines in the inflammatory response showing the broad impact of GR in disease processes.