Abcam, ab223401, Alexa Fluor® 647 Anti-EPF antibody [EPR4476]

Size: 100µL
Rabbit Recombinant Monoclonal EPF antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR4476,
Isotype:IgG,
Conjugation:Alexa Fluor® 647,
Excitation/Emission:Ex: 650nm, Em: 665nm,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
EPF also known as Embryo Paracrine Factor is a protein with a mass of approximately 18-20 kDa. This protein primarily expresses in embryonic tissues and certain adult tissues with regeneration and repair abilities. EPF functions as a signaling molecule and plays a role in cellular communication influencing different cellular behaviors like proliferation differentiation and migration.
Biological function summary
EPF impacts multiple cellular processes during embryogenesis and tissue regeneration. It does not function as a part of a larger protein complex but interacts directly with surface receptors to initiate signal transduction pathways. EPF activities help in regulating cell cycle transitions balancing cell growth with apoptosis for proper tissue formation and healing. By mediating these activities EPF is critical for normal development and efficient repair processes.
Pathways
Several molecular networks engage with EPF to exert its influence. One of the primary pathways involving EPF is the Wnt signaling pathway which contributes to the regulation of gene expression cellular growth and differentiation. Through this pathway EPF interacts with proteins like β-catenin. Additionally EPF also associates with the PI3K/Akt pathway which further highlights its involvement in controlling cellular survival and growth dynamics.
Abnormal EPF function is linked with cancer progression and regenerative disorders. Dysregulation of EPF can lead to uncontrolled cell proliferation and has been observed in certain cancers such as colorectal cancer. Furthermore impaired EPF signaling might affect wound healing processes leading to chronic wounds. During these disease processes EPF interacts with other important signaling molecules such as p53 in cancer to influence disease outcomes.