Abcam, ab223532, Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal PI 3 Kinase catalytic subunit alpha/PIK3CA antibody. Carrier free. Suitable for IP, WB and reacts with Mouse, Human, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR19693,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab223532 is the carrier-free version of
ab183957
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PI 3 kinase catalytic subunit alpha also known as PIK3CA or PI3K-alpha is a protein kinase with significant roles in cellular signaling. This protein has a mass of approximately 124 kDa and is part of the PI3 kinase family. PIK3CA is primarily expressed in various tissues including the liver breast and pancreas where it plays an important role in the regulation of cell growth and survival. The structure of PI3 kinase involves a complex of p110 catalytic subunits and p85 regulatory subunits which facilitate its enzymatic functions.
Biological function summary
The PIK3CA protein phosphorylates the inositol ring of phosphoinositides generating phosphatidylinositol (345)-trisphosphate (PIP3) a lipid second messenger involved in numerous signaling pathways. PIK3CA often acts within a complex interacting with regulatory subunits such as p85 to control its activity. The complex formation enhances its stability and localization to the cell membrane where it can exert its enzymatic actions effectively. In its biological capacity PIK3CA plays a role in cellular proliferation survival and metabolism.
Pathways
The PI3K/Akt signaling pathway involves PIK3CA as an important mediator. This pathway is integral to numerous cellular processes including metabolism growth and survival. PIK3CA links closely with Akt a downstream protein kinase that propagates the signal initiated by PIP3 generation. The mTOR pathway is another critical route involving PIK3CA where it regulates translation and cellular growth. These pathways emphasize the importance of PIK3CA in maintaining cellular homeostasis and responding to external growth signals.
Mutations and aberrations in PIK3CA associate with several cancers especially breast and colorectal cancers. The altered activity of PIK3CA can cause uncontrolled cell proliferation due to constant activation of the PI3K/Akt signaling pathway. This disrupts normal cellular apoptosis and fosters tumor growth. Additionally PIK3CA mutations are linked to certain genetic disorders such as PIK3CA-related overgrowth spectrum (PROS) where overactivation alters normal tissue growth and development. The interaction of PIK3CA with proteins like PTEN a tumor suppressor involved in cancer pathways also highlights its significance in oncogenic processes.