Abcam, ab223896, APC Anti-Myc tag antibody [9E10]

Size: 100µL
Mouse Monoclonal C-Myc/MYC antibody - conjugated to APC. Suitable for Flow Cyt (Intra) and reacts with Tag samples. Cited in 2 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:9E10,
Isotype:IgG1,
Light chain type:kappa,
Conjugation:APC,
Excitation/Emission:Ex: 650nm, Em: 660nm,
Carrier free:No,
Applications:Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Epitope:aa 410-419 of human Myc.,
Specificity:This antibody is specific for Myc tagged proteins. The Myc tag epitope (EQKLISEEDL) is located at the dimerization site of c-myc and therefore this antibody does not perform well at recognizing endogenous c-myc. A publication by Baker AM et al. 2016 (PMID: 26826706 DOI: 10.1111/his.12939) shows the IHC staining generated by the 9E10 clone does not correlate with c-myc mRNA expression.

Properties and Storage Information:
Form-Liquid, Purity-IgG fraction, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Myc tag often referred to as the c-Myc or 9E11 is a synthetic peptide label widely used to facilitate protein purification and detection. It's a short sequence of amino acids with an approximate mass not exceeding 1.2 kDa. Researchers commonly use it because of its easy detection through antibodies like anti-Myc or anti-Myc HRP. This tag is usually fused to proteins of interest in various expression systems including bacterial yeast and mammalian cells.
Biological function summary
The Myc tag plays no direct role in cellular processes but enables the study of tagged proteins in complex biological environments. It doesn’t interfere with the function of the protein it tags and is part of no native cellular complex. Instead it acts as a versatile tool for researchers to monitor protein expression levels subcellular localization and interactions. The Myc tag size facilitates easy integration into experimental designs making it compatible with a wide range of applications.
Pathways
The Myc tag helps map the role of the proteins it is attached to in significant cellular pathways. While the tag itself does not engage in pathways it assists in understanding protein interactions and cellular signaling mechanisms like the MAPK/ERK pathway or the PI3K/Akt pathway. By monitoring tagged proteins scientists can investigate how they interact with other pathway components enabling insights into cellular function and regulation.
The Myc tag aids research into conditions such as cancer and neurodegenerative diseases. The tag itself isn’t linked to any diseases; however it is often used to study proteins involved in these disorders like those in the Myc-oncoprotein family. Researchers use it to examine over-expression or mutation of target proteins connected to disease mechanisms enabling the development of therapeutic approaches and understanding pathogenic processes.