Abcam, ab224047, Anti-Granuphilin antibody

Size: 100µL
Rabbit Polyclonal Granuphilin antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SYTL4 aa 150-350.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human SYTL4 aa 150-350. The exact immunogen used to generate this antibody is proprietary information.Q96C24

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Granuphilin also known as synaptotagmin-like protein 4 (SYTL4) serves as an important player in vesicle trafficking and fusion with its role as a Rab effector protein. It weighs approximately 85 kDa. This protein mainly expresses in insulin-secreting beta cells of the pancreas and in adipocytes. Granuphilin directly binds to Rab3 and Rab27 influencing the movement and docking of secretory granules on the plasma membrane.
Biological function summary
Granuphilin impacts insulin secretion by managing the docking of insulin granules. It is an essential regulator within the secretory pathway. Granuphilin does not work alone but instead associates with a complex of proteins including Rab27A to facilitate precise targeting and fusion of vesicles with cell membranes. This interaction impacts the exocytosis process vital for hormone release.
Pathways
Granuphilin integrates into the insulin secretion pathway and the exocytosis pathway. It acts alongside proteins such as Rab3 influencing the regulation of granule docking and secretion from pancreatic beta cells. By regulating these pathways granuphilin plays a role in glucose homeostasis and helps maintain metabolic balance within the body.
Granuphilin shows a connection to type 2 diabetes and obesity. Disruption in granuphilin functions can lead to impaired insulin secretion contributing the development to type 2 diabetes. Additionally the protein's involvement in adipocyte-related pathways suggests a role in obesity-related mechanisms. Granuphilin interacts with Rab27 in these contexts linking its malfunction to these metabolic disorders.