Abcam, ab224135, Anti-RECQL5 antibody

Size: 100µL
Rabbit Polyclonal RECQL5 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RECQL5 aa 550-700.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human RECQL5 aa 550-700. The exact immunogen used to generate this antibody is proprietary information.O94762

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RECQL5 which is also known as RECQL5 helicase functions as a DNA helicase. It plays roles in unwinding DNAn important for processes like replication repair and recombination. This protein has a molecular mass of approximately 99 kDa. Expression of RECQL5 occurs in various tissues with higher levels found in the brain and testis. Its helicase activity helps maintain genomic stability by resolving DNA structures during cellular replication processes.
Biological function summary
RECQL5 participates in maintaining genome stability by unwinding DNA strands. It does not merely act on its own; RECQL5 is part of complexes involved in DNA repair pathways. This protein interacts with other repair proteins to facilitate proper DNA replication and recombination ensuring the prevention of deleterious mutations and chromosomal abnormalities. Such interactions stabilize genomic integrity which is important for normal cell function and preventing malignancies.
Pathways
RECQL5 is involved in important cellular processes. It plays a significant role in the homologous recombination repair and the non-homologous end joining pathways. In these pathways RECQL5 interacts with proteins like RAD51 and XRCC4 respectively. Through these interactions RECQL5 ensures the accurate repair of DNA double-strand breaks preventing genomic instability and associated disorders.
Mutations or dysregulation of RECQL5 have associations with cancer and Werner syndrome. In cancer cells altered RECQL5 expression can lead to uncontrolled cell division due to faulty DNA repair often involving interactions with tumor suppressor proteins like p53. In Werner syndrome characterized by premature aging RECQL5 and its interactions with WRN protein may affect normal DNA repair and replication processes. Understanding its dysfunction aids in comprehending these conditions' pathogenesis and potential therapeutic targets.