Abcam, ab224265, Anti-MMP8 antibody [EP1252Y] - Low endotoxin, Azide free

Size: 100µg / 1mg
Rabbit Monoclonal MMP8 antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human, Rat, Mouse samples. Cited in 10 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP1252Y,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WB, IP, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:WB positive for Human recombinant MMP8 but negative results for the following cell lines and tissue: HeLa, A431,K562 and bone marrow.

Product details:
ab224265 is the carrier-free version of
ab81286
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.
What does low endotoxin mean?
Our low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Matrix metalloproteinase-8 (MMP-8) also known as neutrophil collagenase is an enzyme with a molecular weight of approximately 57 kDa. It belongs to the matrix metalloproteinase family which is involved in the breakdown of extracellular matrix components. MMP-8 mainly expresses in neutrophils but can also be found in other tissues such as the skin lungs and liver. The expression of MMP-8 increases during inflammation and infection where it plays a critical role in tissue remodeling and repair processes.
Biological function summary
MMP-8 participates in the degradation of collagen types I II and III contributing to extracellular matrix remodeling. It acts independently rather than being part of a larger enzyme complex. Its activity is tightly regulated by tissue inhibitors of metalloproteinases (TIMPs) to prevent excessive tissue damage. By modulating the extracellular matrix MMP-8 influences processes like wound healing cell migration and angiogenesis indicating its essential role in maintaining tissue homeostasis.
Pathways
MMP-8 plays a role in the inflammatory response and wound healing pathways. It interacts with and processes other proteins like pro-inflammatory cytokines and growth factors which are important for regulating these pathways. MMP-8 collaborates with matrix metalloproteinase-9 (MMP-9) in the inflammatory pathway to facilitate leukocyte migration and rapid tissue remodeling during acute inflammatory responses. Its regulation involves complex interactions among proteolytic enzymes emphasizing its function in balancing matrix degradation and formation.
MMP-8 is associated with periodontal disease and rheumatoid arthritis. In periodontal disease elevated MMP-8 levels can lead to breakdown of connective tissue and bone contributing to tooth loss. In rheumatoid arthritis MMP-8 alongside MMP-1 degrades joint cartilage collagen exacerbating the inflammation and joint damage. These associations underline MMP-8's potential as a biomarker for these diseases and highlight the need for MMP-8 testing tools such as MMP8 ELISA kits to monitor disease progression and treatment efficacy.