Abcam, ab224424, Anti-PPM1K antibody

Size: 100µL
Rabbit Polyclonal PPM1K antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PPM1K aa 250 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human PPM1K aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q8N3J5

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PPM1K also known as Protein Phosphatase Mg2+/Mn2+ Dependent 1K is a mitochondrial phosphatase with a molecular weight of approximately 42 kDa. It is expressed in a variety of tissues with notable expression in the liver and skeletal muscle. PPM1K plays a critical role in regulating various cellular processes through the dephosphorylation of specific substrates involved in metabolic pathways.
Biological function summary
PPM1K influences mitochondrial function and energy metabolism by modulating branch-chained amino acid (BCAA) catabolism. It forms part of a metabolically linked complex that regulates the enzymatic activity essential for breaking down BCAAs into vital intermediates for energy production. The modulatory effect of PPM1K on these pathways highlights its importance in maintaining cellular energy balance and metabolic homeostasis.
Pathways
PPM1K contributes significantly to the regulation of the branched-chain alpha-keto acid dehydrogenase (BCKDH) complex which is important in the catabolic pathway of BCAAs. By dephosphorylating and activating BCKDH PPM1K facilitates the breakdown of leucine isoleucine and valine. PPM1K’s interaction with components of the BCKDH complex links it to broader metabolic processes and connects it functionally to other enzymes responsible for maintaining amino acid homeostasis.
PPM1K has connections to disorders related to energy imbalance and mitochondrial dysfunctions such as Maple Syrup Urine Disease (MSUD) and diabetes. Deficiencies or mutations in the PPM1K gene influence the stability and function of the BCKDH complex leading to impaired BCAA catabolism. This impairment results in the accumulation of toxic metabolites contributing to the pathophysiology of MSUD. PPM1K’s regulation of BCKDH activity also indirectly connects it to enzymes involved in gluconeogenesis and energy production highlighting its broad relevance in metabolic disorders.