Abcam, ab224435, Anti-NPL4 antibody

Size: 100µL
Rabbit Polyclonal NPL4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NPLOC4 aa 350-550.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human NPLOC4 aa 350-550. The exact immunogen used to generate this antibody is proprietary information.Q8TAT6

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein NPL4 known also as Nuclear Protein Localization 4 is involved in key cellular processes as a cofactor in the p97 ATPase complex. It has a molecular mass of approximately 68 kDa. NPL4 is expressed in different tissues with increased levels observed in dividing cells. Mechanically NPL4 aids in protein degradation by advancing the extraction of ubiquitinated substrates from the ER-associated degradation (ERAD) pathway.
Biological function summary
NPL4 participates in the unfoldase activity required for the ERAD process. It functions within a complex that includes p97/VCP and other cofactors like UFD1. This complex targets misfolded proteins for degradation in the proteasome maintaining cellular proteostasis. In a broader scope NPL4 ensures efficient cellular response to protein stress which is necessary for normal cell cycle progression and mitosis.
Pathways
NPL4 integrates into essential proteostasis pathways interplaying with ERAD and the ubiquitin-proteasome system. It interacts with key proteins like UFD1 and p97/VCP within these pathways. The NPL4-p97-VCP complex facilitates protein dislocation during ER stress therefore influencing the unfolded protein response (UPR) a critical cellular adaptation mechanism.
Malfunction of NPL4 has links to neurodegenerative diseases and certain cancers. Impairments in NPL4 function and its complex with p97/VCP associate with the accumulation of ubiquitinated proteins which contributes to pathological states such as amyotrophic lateral sclerosis (ALS) and various tumor progressions. Mutations within proteins of this complex also play a role in those conditions underpinning the critical importance of NPL4 in cellular health.