Abcam, ab22453, Anti-CDKL5 antibody

Size: 100µg
Rabbit Polyclonal CDKL5 antibody. Suitable for WB and reacts with Mouse samples. Cited in 7 publications.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Swissprot predicts mouse CDKL5 protein to be ~105 kDa (Q3UTQ8), however a recent paper by Lin et al, 2006 (PMID: 16330482) indicated CDKL5 to run at ~118 kDa on WB. On this basis we think that the ~120 kDa band we observe using Rabbit polyclonal to CDKL5 (ab22453) on mouse tissue is CDKL5 specific. Futhermore the ~120 kDa band is blocked by the immunising peptide. The larger ~200 kDa is potentially also CDKL5 as a similar band is also observed in the Lin et al (2006) publication. There is evidence for larger forms of CDKL5 for the human version of the protein, so it may not be surprising to find a similar scenario for the mouse protein.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle