Abcam, ab22501, Anti-Respiratory Syncytial Virus Nucleoprotein antibody [B023]

Size: 100µL
Mouse Monoclonal NCAP antibody. Suitable for ELISA, sELISA, IHC-Fr and reacts with Respiratory syncytial virus samples. Cited in 5 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:B023,
Isotype:IgG1,
Carrier free:No,
Reacts with:Respiratory syncytial virus,
Applications:sELISA, ELISA, IHC-FrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:This antibody recognises the 42kD RSV nucleo protein.

Properties and Storage Information:
Form-Liquid, Purification notes-Protein A chromatography, Storage buffer-Preservative: 0.09% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Respiratory Syncytial Virus Nucleoprotein (RSV N protein) plays a central mechanical role in the replication and transcription of the RSV genome. It binds single-stranded RNA acting as a scaffold for the virus's RNA synthesis. The nucleocapsid protein another name for RSV N protein has a molecular mass of approximately 45 kilodaltons. It is expressed in cells infected by the RSV notably within the cytoplasm forming a protective helix around the viral RNA genome.
Biological function summary
Within the context of viral infection and replication RSV N protein is essential for packaging and protecting the viral RNA genome. It forms part of a complex with the phosphoprotein (P protein) and the large polymerase protein (L protein) to facilitate RNA replication and transcription. This complex ensures the stability and integrity of the viral genome allowing the virus to efficiently replicate and produce infectious particles. Therefore the nucleoprotein strongly influences the life cycle of the virus.
Pathways
The RSV N protein functional capacity is pivotal within the viral RNA transcription and replication pathways. It closely interacts with the phosphoprotein P enhancing the activity of the viral RNA-dependent RNA polymerase complex. This interaction synergistically ties into the virus's replication pathway ensuring the production of viral RNAs. The N protein also coordinates with M2-1 protein in regulating mRNA synthesis and RNA processing reflecting its comprehensive involvement in essential viral pathways.
The involvement of RSV N protein connects strongly to respiratory tract infections particularly in infants and the elderly. RSV infection often leads to bronchiolitis and pneumonia where the N protein plays an important role in propagating the disease. Its interaction with the fusion glycoprotein (F protein) facilitates virus assembly and release which enhances viral spread and subsequent infection severity. Researchers often focus on these proteins when developing therapeutics targeting RSV-related diseases.