Abcam, ab225877, Anti-TSG101 antibody

Size: 100µL
Rabbit Polyclonal TSG101 antibody. Suitable for WB and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human Tumor susceptibility gene 101 protein aa 200-300.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human Tumor susceptibility gene 101 protein aa 200-300. The exact immunogen used to generate this antibody is proprietary information.Q99816

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab225877 was affinity purified using an epitope specific to TSG101 immobilized on solid support., Storage buffer-pH: 6.8 - 7.4Preservative: 0.09% Sodium azideConstituents: Tris buffered saline, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TSG101 also known as Tumor Susceptibility Gene 101 is a protein involved in intracellular trafficking specifically notable within the process of endosomal sorting. It is a component of the ESCRT-I (Endosomal Sorting Complex Required for Transport-I) complex. TSG101 is approximately 46 kDa and is expressed ubiquitously across various tissues. The mechanistic function of TSG101 involves the recognition and ubiquitination of cargo proteins an important step in the formation of multivesicular bodies.
Biological function summary
TSG101 plays a critical role in the sorting and trafficking of proteins destined for lysosomal degradation or recycling back to the plasma membrane. It is an essential part of the ESCRT machinery which also includes other components like VPS37 and VPS28. TSG101 interacts with specific domains and motifs on target proteins facilitating the recognition and recruitment of these proteins into vesicles. Its involvement in membrane budding and scission makes it important for maintaining cellular homeostasis and protein quality control.
Pathways
TSG101 is integrally involved in the ESCRT pathway and is associated with processes like viral budding and cytokinesis. Within these pathways it collaborates with proteins such as ALIX and the ESCRT-II component VPS25 to mediate vesicle formation and facilitate the trafficking of ubiquitinated cargo. The ESCRT pathway is vital in cellular responses such as membrane repair and is important for the life cycle of certain enveloped viruses which utilize host cell machinery for budding.
Defects or alterations in TSG101 expression have correlations with tumor progression and neurodegenerative diseases. Disruptions in the function of TSG101 can affect cellular processes potentially contributing to conditions like cancer and Alzheimer's disease. For example the aberrant activity of TSG101 may interact with proteins like Hrs and ubiquitin ligases Mdm2 in the context of oncogenic signaling pathways which can drive tumor growth and metastasis. Understanding these interactions is key to uncovering potential therapeutic targets within these diseases.