Abcam, ab225887, Anti-SENP1 antibody

Size: 100µL
Rabbit Polyclonal SENP1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SENP1 aa 1-50.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human SENP1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.Q9P0U3

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab225887 was affinity purified using an epitope specific to SENP1 immobilized on solid support., Storage buffer-pH: 6.8 - 7.4Preservative: 0.09% Sodium azideConstituents: Tris buffered saline, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SENP1 also known as SUMO-specific protease 1 is an enzyme involved in the process of sumoylation a post-translational modification mechanism affecting various proteins. This enzyme has an approximate molecular mass of 73 kDa. SENP1 specifically cleaves SUMO (Small Ubiquitin-like Modifier) from target proteins regulating their function and localization. It shows strong expression in tissues with high cellular turnover like the thymus spleen and testis indicating its significant role in cell growth and development processes.
Biological function summary
SENP1 contributes to cellular homeostasis by modifying and controlling protein interactions and functions. This enzyme facilitates the recycling of SUMO proteins and affects transcription factors such as HIF1α which are vital for cellular response to hypoxia. SENP1 interacts closely with SUMO E3 ligases in complexes coordinating the addition or removal of SUMO groups from target proteins. Its activity is essential for maintaining the balance between protein sumoylation and desumoylation which impacts numerous cellular processes including cell cycle progression and stress response.
Pathways
SENP1 modulates key regulatory pathways related to its sumoylation mechanism. Major pathways where SENP1 plays a role include the HIF-1 signaling pathway where it affects the stability and activity of HIF1α under low oxygen conditions and the p53 signaling pathway influencing cell cycle and apoptosis. SENP1's interactivity with proteins like Ubc9 a SUMO-conjugating enzyme and PIAS3 a SUMO E3 ligase indicates its broad involvement and regulation within these pathways emphasizing its impact on maintaining cellular function and adaptation.
SENP1 associates with certain cancers and neurodegenerative disorders due to its regulatory influence on protein stability and gene expression. Overexpression of SENP1 is often observed in prostate cancer where it desumoylates HIF1α enhancing its transcriptional activity and promoting tumorigenesis. Additionally alterations in SENP1 function might connect to neurodegenerative diseases like Alzheimer's where protein turnover and cellular stress responses are disrupted. Through these disorders SENP1 connects with proteins such as AR (androgen receptor) in cancer contexts and Tau in Alzheimer's underlying its role in disease pathology.