Abcam, ab226119, Anti-ACTL6A antibody [EPR7443] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal ACL6A antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR7443,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:The immunogen used for this product shares 79% homology with ACTL6B. Cross-reactivity with this protein has not been confirmed experimentally.

Product details:
ab226119 is the carrier-free version of
ab131272
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ACTL6A also known as BAF53A or Arp4 is a member of the actin-related protein family with a molecular mass of approximately 53 kDa. This protein plays an important role in chromatin remodeling facilitating transcriptional regulation by altering nucleosome positioning. ACTL6A shows expression in various tissues with high levels found in neurons and certain cancer cell types. Its function depends on local cellular context which influences its involvement in specific gene expression profiles.
Biological function summary
ACTL6A supports transcriptional regulation by integrating into the SWI/SNF chromatin-remodeling complex. This complex which includes other key proteins coordinates to reposition nucleosomes aiding transcription factors in accessing target DNA sequences. ACTL6A contributes to regulation of gene expression patterns that underline cell identity and differentiation reflecting its critical role in neuronal and cancer cell biology.
Pathways
The SWI/SNF complex which includes ACTL6A integrates into pathways that control cell proliferation and differentiation. Specifically the Wnt signaling pathway uses ACTL6A to regulate gene expression necessary for proper cell function. Proteins like BRG1 and BRM serve as partners in this process helping to execute transcriptional activation. By participating in these pathways ACTL6A emphasizes its role in conserving cellular homeostasis.
ACTL6A exhibits associations with oncogenesis and neural disorders. Researchers observed its link to the development of glioblastoma where it influences tumor progression through interactions with other SWI/SNF members like SMARCA4. Additionally abnormalities in ACTL6A function relate to neurodevelopmental disorders emphasizing its importance in neuronal growth and differentiation. Its connection to these diseases highlights the potential of ACTL6A as a target for therapeutic interventions.