Abcam, ab226195, Anti-Ran antibody

Size: 100µL
Rabbit Polyclonal RAN antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human RAN aa 150 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, IP, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human RAN aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.P62826

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab226195 was affinity purified using an epitope specific to Ran immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Ran also known as Ras-related nuclear protein is a small GTP-binding protein with a mass of approximately 24 kDa. It localizes mainly in the nucleus and cytoplasm of cells and is highly expressed in actively dividing cells including various types of tumors. Ran plays a mechanical role in nucleocytoplasmic transport by facilitating the transport of proteins and ribonucleic acids through the nuclear pore complex. This protein belongs to the Ras superfamily of small GTPases which cycle between active GTP-bound and inactive GDP-bound states.
Biological function summary
Ran is a central part of the nucleocytoplasmic transport complex and directly participates in the regulation of genome organization and cell cycle progression. Ran's GTP-bound state helps maintain nuclear import and export balance supporting chromatin condensation and mitotic spindle assembly. This protein's complex involvement in the chromosomal region maintenance highlights its importance in cellular homeostasis emphasizing its role in organisms' growth and development.
Pathways
The mechanical functions of Ran impact the Ran GTPase pathway known for regulating nucleocytoplasmic transport and mitotic spindle organization. In the Ran cycle the protein works closely with RCC1 its guanine nucleotide exchange factor and RanGAP1 the GTPase-activating protein. These interactions are essential during cell division particularly for spindle assembly. Ran also aligns with pathways involving importins and exportins affecting protein export and nuclear import rates vital for cellular cyclic processes.
Ran has implications in cancer and neurodegenerative conditions. The protein's overexpression or dysfunction often correlate with various cancers due to its role in cell division and genetic material organization making it a focal point for cancer research. Additionally changes in Ran's regulatory pathways may influence neurodegenerative diseases by affecting nucleocytoplasmic transport in neurons. In these disorders Ran interacts with proteins such as CRM1 and others involved in the transport machinery highlighting its potential as a therapeutic target.