Abcam, ab227011, Anti-RPS27A antibody

Size: 100µL
Rabbit Polyclonal RPS27A antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RPS27A.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human RPS27A. The exact immunogen used to generate this antibody is proprietary information.P62979

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.01% Thimerosal (merthiolate)Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RPS27A also known as Ubiquitin-40S Ribosomal Protein S27a is a fusion protein consisting of ubiquitin and ribosomal protein S27a. It has a molecular mass of approximately 17 kDa. RPS27A is expressed in multiple tissues but has higher expression in actively dividing cells where protein synthesis is high. Mechanically RPS27A contributes to two different functions: ubiquitin marks proteins for degradation while the S27a component incorporates into the 40S ribosomal subunit playing a role in the assembly and function of the ribosome.
Biological function summary
RPS27A serves dual roles due to its hybrid nature. It participates in protein homeostasis through the ubiquitin-proteasome system by tagging proteins for degradation. Additionally it is an essential component of the ribosomal 40S subunit forming part of the cellular machinery that translates mRNA into polypeptides. This dual role allows RPS27A to impact general cell function linking protein turnover with the protein production machinery.
Pathways
RPS27A contributes to the ubiquitin-mediated proteolysis pathway and the ribosome biogenesis pathway. In ubiquitin-mediated proteolysis RPS27A partners with proteins like E1 E2 and E3 enzymes which aid in the transfer and attachment of ubiquitin to target proteins. In ribosome biogenesis RPS27A's ribosomal component joins with other ribosomal proteins within the nucleolus to form the mature ribosome integral to protein synthesis.
RPS27A appears to have associations with cancer and neurodegenerative diseases. In cancer abnormalities in ubiquitin-mediated proteolysis may lead to unchecked cell division implicating RPS27A in tumorigenesis. Additionally disruptions in ribosomal biogenesis to which RPS27A contributes may also play a role in cancer development. Moreover dysfunction in protein degradation mechanisms involving ubiquitin has links to neurodegenerative disorders such as Parkinson’s disease where an accumulation of misfolded or damaged proteins occurs. In such contexts proteins interacting with RPS27A like ubiquitin ligases or proteasomes may be directly involved.