Abcam, ab227413, Recombinant human Aldolase C protein

Size: 5µg
Recombinant human Aldolase C protein is a Human Full Length protein, in the 1 to 364 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, FuncS, Mass Spec.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Escherichia coli,
Tags:Tag free,
Applications:FuncS, Mass Spec, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Specific activity is > 6 units/mg. One unit will convert 1.0 μmol of fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehydes 3- phosphate per minute at pH 7.5 at 37°C.,
Accession:P09972,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Aldolase C also known as ALDOC is an enzyme that plays an important role in glycolysis. It catalyzes the reversible conversion of fructose-16-bisphosphate into dihydroxyacetone phosphate and glyceraldehyde-3-phosphate. Aldolase C has a molecular mass of approximately 39 kDa. This enzyme is primarily expressed in the brain particularly in astrocytes where it serves as an identifier for these cells.
Biological function summary
This enzyme contributes to energy metabolism within cells notably astrocytes. It is part of the aldolase family which includes isoenzymes like aldolase A and B that perform similar functions in different tissues. Aldolase C is involved in maintaining glucose homeostasis and energy production in the brain where it forms part of a complex with other glycolytic enzymes to facilitate efficient cellular respiration.
Pathways
Aldolase C is a significant component of the glycolytic pathway facilitating energy production in cells. It plays alongside other glycolytic enzymes such as hexokinase phosphofructokinase and pyruvate kinase to convert glucose into pyruvate transferring energy in the process. Additionally aldolase C participates in the fructose metabolism pathway similar to its isoenzymes aiding in the conversion of fructose derivatives important for maintaining normal energy levels in brain tissues.
Aldolase C has connections to neurological conditions like glioma and neurodegenerative diseases. Altered expression levels of aldolase C can indicate glioma progression or serve as a biomarker in astrocytic neurodegenerative disorders. The enzyme’s function is also linked to its interaction with other proteins such as Glial Fibrillary Acidic Protein (GFAP) in astrocytes which assist in maintaining normal cell structure and response to damage. Therefore aldolase C serves both as a metabolic enzyme and a potential marker in assessing neurological health and disease.